Development and applications of simultaneous immunochemical staining and serial detection of overlapping proteins in blotting procedures

► Detection of overlaying proteins by serial peroxidase conjugated antibody addition. ► Differentiation of proteins with identical molecular masses on one immunoblot. ► Serial antigen determinations in complex protein suspensions using dot blotting. ► Inactivation of antibody conjugated peroxidase by hydrogen peroxide incubation.