Purification of functional eosinophils from human bone marrow

► Novel methodology for the purification of eosinophils from human bone marrow. ► Marrow- and blood-eosinophils exhibit similar activation kinetics. ► Cultured blood- and marrow-eosinophils show chemotaxis toward CXCL12. ► Marrow-eosinophils, unlike blood-eosinophils, express surface CXCR4.