A broadly applicable approach to T cell epitope identification: Application to improving tumor associated epitopes and identifying epitopes in complex pathogens

► Developed a method to identify T cell epitopes, critical tools for immunology. ► Uses bacterial expression libraries and solid-phase antigen delivery. ► Identified MHC class I restricted altered epitopes with super-agonist activities. ► Showed feasibility of screening expression library constructed from F. tularensis. ► Identified epitopes in Francisella pathogenicity proteins using new Gateway vector.