Reporter gene assay for the quantification of the activity and neutralizing antibody response to TNFα antagonists

► TNFα responsive reporter cells can quantify TNFα activity within 2 hours. ► Reporter cells can quantify anti-TNFα drug activity in human serum. ► Reporter cells can quantify neutralizing antibodies to anti-TNFα drugs in serum. ► Results independent of sample cytotoxicity or serum matrix effects.