کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8419760 | 1545752 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Yeast surface display of antibodies via the heterodimeric interaction of two coiled-coil adapters
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کلمات کلیدی
PBSFACSscFvscFv antibodiesSingle chain Fv fragmentSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریYeast display - نمایش مخمر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Yeast surface display of antibodies via the heterodimeric interaction of two coiled-coil adapters Yeast surface display of antibodies via the heterodimeric interaction of two coiled-coil adapters](/preview/png/8419760.png)
چکیده انگلیسی
A novel adapter-directed yeast display system with modular features was developed. This display system consists of two modules, a display vector and a helper vector, and is capable of displaying proteins of interest on the surface of Saccharomyces cerevisiae through the interaction of two small adapters that are expressed from the display and helper vectors. In this report, an anti-VEGF scFv antibody gene was cloned into the display vector and introduced alone into yeast S. cerevisiae cells. This led to the expression and secretion of a scFv antibody that was fused in-frame with the coiled-coil adapter GR1. For display purposes, a helper vector was constructed to express the second coiled-coil adapter GR2 that was fused with the outer wall protein Cwp2, and this was genetically integrated into the yeast genome. Co-expression of the scFv-GR1 and GR2-Cwp2 fusions in the yeast cells resulted in the functional display of anti-VEGF scFv antibodies on the yeast cell surfaces through pairwise interaction between the GR1 and GR2 adapters. Visualization of the co-localization of GR1 and GR2 on the cell surfaces confirmed the adapter-directed display mechanism. When the adapter-directed phage and yeast display modules are combined, it is possible to expand the adapter-directed display to a novel cross-species display that can shuttle between phage and yeast systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 354, Issues 1â2, 31 March 2010, Pages 11-19
Journal: Journal of Immunological Methods - Volume 354, Issues 1â2, 31 March 2010, Pages 11-19
نویسندگان
Kevin Caili Wang, Chirag A. Patel, Jian Wang, Jinqing Wang, Xinwei Wang, Peter Peizhi Luo, Pingyu Zhong,