کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8419776 1545752 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
T-cell enumeration from dried blood spots by quantifying rearranged T-cell receptor-β genes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
T-cell enumeration from dried blood spots by quantifying rearranged T-cell receptor-β genes
چکیده انگلیسی
Significant hurdles remain to large-scale implementation of medical interventions in the developing world due to the lack of a modern diagnostic infrastructure. This is especially pertinent to the international roll-out of antiretroviral drugs to treat HIV, which ideally includes a CD4 T-cell count to determine eligibility. We designed a novel technique to estimate mature T-cell numbers by calculating the amount of rearranged T-cell receptor β genes from dried blood spots of HIV-infected individuals in the United States and Uganda. It was observed that the rearranged T-cell receptor β count correlated well with total lymphocyte counts from both study populations (Baltimore R = 0.602, Uganda R = 0.497; p < 0.001) and the ability for this measurement to determine antiretroviral initiation was similar to total lymphocyte counts, which can be used to determine eligibility in HIV + children. This technique as well as other dried blood spot based technologies could increase the diagnostic and monitoring capabilities in resource-limited settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 354, Issues 1–2, 31 March 2010, Pages 40-44
نویسندگان
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