A high-throughput technique to quantify bacterial pathogens' virulence on the insect model Galleria mellonella

We combined spectrophotometry and an original statistical approach to infer bacteria virulence, using the lepidoptera Galleria mellonella as a host model. With this method, it is possible to use a microplate reader to automatize data collection on host survival on batches of 96 samples. The method also allows measurement of pathogen multiplication if GFP labelled bacterial strains are used.