کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8420273 | 1545891 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation of multi-metal tolerant ubiquitin fusion protein from metal polluted soil by metatranscriptomic approach
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Isolation of multi-metal tolerant ubiquitin fusion protein from metal polluted soil by metatranscriptomic approach Isolation of multi-metal tolerant ubiquitin fusion protein from metal polluted soil by metatranscriptomic approach](/preview/png/8420273.png)
چکیده انگلیسی
Release of heavy metals into the soil pose a significant threat to the environment and public health because of their toxicity accumulation in the food chain and persistence in nature. The potential of soil microbial diversity of cadmium (Cd) contaminated site was exploited through functional metatranscriptomics by construction of cDNA libraries A (0.1-0.5â¯kb), B (0.5-1.0â¯kb), and C (1-4â¯kb) of variable size, from the eukaryotic mRNA. The cDNA library B was further screened for cadmium tolerant transcripts through yeast complementation system. We are reporting one of the transformants ycf1ÎPLBe1 capable of tolerating high concentrations of Cd (40â¯Î¼M - 80â¯Î¼M). Sequence analysis revealed that PLBe1 cDNA showed homology with ubiquitin domain containing protein fused with AN1 type zinc finger protein of Acanthameoba castellani. Further, this cDNA was tested for its tolerance towards other heavy metals such as copper (Cu), zinc (Zn) and cobalt (Co). Functional complementation assay of cDNA PLBe1 showed a range of tolerance towards copper (150â¯Î¼M - 300â¯Î¼M), zinc (10â¯mM - 12â¯mM) and cobalt (2â¯mM - 4â¯mM). This study promulgates PLBe1 as credible member of multi-metal tolerant gene in the eukaryotic soil microbial community and can be used as potential member to revitalise the heavy metal contaminated sites or can be used as a biomarker to detect heavy metal contamination in the soil environment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 152, September 2018, Pages 119-125
Journal: Journal of Microbiological Methods - Volume 152, September 2018, Pages 119-125
نویسندگان
Bharti Thakur, Rajiv Yadav, Laurence Fraissinet-Tachet, Roland Marmeisse, M. Sudhakara Reddy,