کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8420285 | 1545891 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy Directed freeze-fracturing of Bacillus subtilis biofilms for conventional scanning electron microscopy](/preview/png/8420285.png)
چکیده انگلیسی
Biofilms have been intensively investigated over the past decades. Bacillus subtilis is able to form highly structured colony biofilms, and as one of the most studied Gram-positive model organisms, has helped to decipher the complex genetic regulation of biofilms. Several methods have been developed to analyze the architecture of biofilms. In this paper, we describe a method which allows the analysis of the internal structures of biofilms by scanning electron microscopy (SEM). The method uses a modified freeze-fracturing of chemically fixed biofilm to generate defined, well-preserved fractures at millimeter-scale which allows to analyze systematically the internal biofilm structure from macro- to nano-scale.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 152, September 2018, Pages 165-172
Journal: Journal of Microbiological Methods - Volume 152, September 2018, Pages 165-172
نویسندگان
Felix M. Fuchs, Gudrun Holland, Ralf Moeller, Michael Laue,