A senescence-delaying pre-culture medium for transcriptomics of Podospora anserina

Podospora anserina is an efficient degrader of recalcitrant plant biomass but senesces quickly on most standard pre-culturing media. Among nine pre-culture media, sufficient growth without senescence was only observed on Luria-Bertani medium. The high quality RNA obtained from subsequent transfer cultures was suitable for transcriptomics.