کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8421016 1545918 2016 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Discriminative power of Campylobacter phenotypic and genotypic typing methods
ترجمه فارسی عنوان
قدرت تشخیصی روش های تایپ آمیزه های ژنوتیپ و فنوتیپ های کمپیلوباکتر
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی
The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n = 94) and Campylobacter coli (n = 52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (MLST), flagellin gene A restriction fragment length polymorphism typing (flaA-RFLP), antimicrobial resistance profiling (AMRp), the presence/absence of 5 putative virulence genes; and, exclusively for C. jejuni, the determination of lipooligosaccharide (LOS) class. Discriminatory power was calculated by the Simpson's index of diversity (SID) and the congruence was measured by the adjusted Rand index and adjusted Wallace coefficient. MLST was individually the most discriminative typing method for both C. jejuni (SID = 0.981) and C. coli (SID = 0.957). The most discriminative combination with a SID of 0.992 for both C. jejuni and C. coli was obtained by combining MLST with flaA-RFLP. The combination of MLST with flaA-RFLP is an easy and feasible typing method for short-term monitoring of Campylobacter in broiler meat carcass.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 125, June 2016, Pages 33-39
نویسندگان
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