A new biphasic test for the detection of Helicobacter pylori in gastric biopsies

The objective of this study was to create a biphasic cultural method for the detection of Helicobacter pylori in gastric biopsy specimens. The biphasic systems were made by using a urea agar slant with overlaying broth in a single vessel. Initially, three different liquid media including brain-heart infusion broth, Brucella broth, and Bolton broth were tested for their ability to support the growth of H. pylori. Bolton broth with 10% defibrinated horse blood demonstrated a significant increase in the numbers of H. pylori (p < 0.05). The result showed that positive urease was used to concentrate viable H. pylori cells where the numbers of bacteria were 105 cfu. In addition, the reliable incubation time was at least 36 h. In total, 55 biopsies were comparatively studied using commercial rapid urease test and PCR. Seven samples (12.72%) were positive with H. pylori by the biphasic test. With the CLOtest, 6 (10.91%) samples were positive. In conclusion, the Hp biphasic test achieved more positive samples than did the commercial rapid urease test.