کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8425183 | 1546024 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effects of plasma gelsolin on human erythroblast maturation for erythrocyte production
ترجمه فارسی عنوان
اثر گلیسولین پلاسما بر بلوغ انسانی اریتروبلاست برای تولید اریتروسیت
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کلمات کلیدی
VLA-4GATA-binding factor 1DLC-1ICAM-4GSNHSCsCDKRhoAGAPDHRed blood cells - سلولهای قرمز خونErythropoiesis - erythropoiesisvery late antigen-4 - خیلی زود antigen-4Dysplasia - دیسپلازی hematopoietic stem cells - سلول های بنیادی خونسازRas homolog gene family member A - عضو خانواده خانواده ژن رای همولوگGelsolin - ژلزولینcyclin-dependent kinase - کییناز وابسته به سیکلینGATA-1 - گاتا-1glyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
Gelsolin is an actin binding protein present in blood plasma and in cytoplasm of cells including macrophages. Gelsolin has important functions in cell cycle regulation, apoptotic regulation, and morphogenesis. Even though bone marrow macrophages and serum factors are critical for regulating erythropoiesis, the role of gelsolin on human erythroblasts has not been studied. Here, we investigated the effects of human recombinant plasma gelsolin (pGSN) on human immature erythroblasts. CD34+ cells isolated from cord blood were differentiated into erythroid cells in serum-free medium. When pGSN was applied to the culture medium, it accelerated basophilic and polychromatic erythroblast maturation and increased the enucleation rate with highly expressed erythropoiesis-related mRNAs. Also, pGSN was effective in reducing dysplastic changes caused by vincristine, suggesting its role in cell cycle progression at G2/M checkpoints. Also, pGSN activated caspase-3 during maturation stages in which caspase-3 functions as a non-apoptotic maturational signal or a pro-apoptotic signal depending on maturation stages. Our results suggest that pGSN has a pivotal role in maturation of erythroblasts and this factor might be one of the way how bone marrow macrophages and previously unknown serum factors work to control erythropoiesis. pGSN might be used as additive for in vitro production of erythrocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 29, May 2018, Pages 64-75
Journal: Stem Cell Research - Volume 29, May 2018, Pages 64-75
نویسندگان
So Yeon Han, Eun Mi Lee, Hye Sook Choi, Bok Hwan Chun, Eun Jung Baek,