کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8426635 | 1546053 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interval of gonadotropin administration for in vitro embryo production from oocytes collected from Holstein calves between 2 and 6 months of age by repeated laparoscopy
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Laparoscopic Ovum Pick-Up (LOPU) in calves followed by in vitro embryo production (IVEP) and transfer (ET) into adult recipients has great potential for accelerated genetic gain through shortening of the generation interval. In this study, 11 Holstein calves were subjected to up to six LOPU procedures between the ages of 2-6â¯monthsâ¯at 2-3 weeks interval. In all cases, the animals received a CIDR 5 days prior to LOPU and were gonadotropin-stimulated starting at 72â¯h before LOPU using one of three protocols that were rotated twice among the animals during the study. Calves were injected with FSH every 12â¯h (FSH12h), or every 8â¯h (FSH8h) or every 8â¯h until â36â¯h from LOPU at which point the FSH was replaced with a single dose of 400 IU eCG (FSH8h-eCG). No statistical differences were observed among the 3 treatments in terms of mean follicles available for aspiration (35.7â¯Â±â¯16 vs. 38.5â¯Â±â¯25 vs. 31.1â¯Â±â¯22), mean oocytes recovered (26.5â¯Â±â¯14 vs. 21.6â¯Â±â¯10 vs. 19.4â¯Â±â¯14) and cleavage rate (66.0â¯Â±â¯14 vs. 61.1â¯Â±â¯11 vs. 72.2â¯Â±â¯8), for FSH12h, FSH8h and FSH8h-eCG, respectively. However, FSH8h-eCG resulted in a significantly higher rate of transferable embryos (17.5â¯Â±â¯8%) compared with FSH12h (8.9â¯Â±â¯5%, Pâ¯<â¯0.05). Oocytes from follicles of â¥5â¯mm in diameter yielded a higher rate (Pâ¯<â¯0.05) of development to the blastocyst stage (13.8%) than those collected from <5â¯mm follicles (6.8%). Animal age, by comparing animals at <100, 101 to 130 andâ¯>â¯130 days of age, did not affect the mean number of follicles (34.2â¯Â±â¯15 vs. 39.3â¯Â±â¯26 vs. 31.6â¯Â±â¯25), the mean number of oocytes recovered (21.2â¯Â±â¯10 vs. 24.5â¯Â±â¯15 vs. 22.6â¯Â±â¯17), and the cleavage rate (68.6â¯Â±â¯11 vs. 61.7â¯Â±â¯12 vs. 70.7â¯Â±â¯10%), respectively. However, animals in the older age range had significantly higher development to the blastocyst stage (19.9â¯Â±â¯6 vs. 9.5â¯Â±â¯8%, Pâ¯<â¯0.01) and better embryo quality, as evidenced by higher average cell numbers (119.1â¯Â±â¯47 vs. 91.5â¯Â±â¯25, Pâ¯<â¯0.05) compared with those in the lower age. Finally, we tested the benefits of relieving endoplasmic reticulum stress by supplementing the culture medium with 50â¯Î¼M tauroursodeoxycholic acid (TUDCA) and found a numerically higher rate of development to the blastocyst stage (21.1â¯Â±â¯8 vs. 18.6â¯Â±â¯4%), but not statistically different, compared with control culture. Overall, our findings indicate that a significant number of transferable embryos (range 10-30) can be produced from Holstein calves before they reach 6 months of age.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 116, August 2018, Pages 64-70
Journal: Theriogenology - Volume 116, August 2018, Pages 64-70
نویسندگان
Hernan Baldassarre, Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Rafael G. Mondadori, Werner G. Glanzner, Yasmin Schuermann, Rodrigo C. Bohrer, Naomi Dicks, Rosalba Lopez, François-Xavier Grand, Christian Vigneault,