کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8427100 | 1546055 | 2018 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of voltage-gated potassium channel (Kv) and its importance in functional dynamics in bull spermatozoa
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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![عکس صفحه اول مقاله: Molecular characterization of voltage-gated potassium channel (Kv) and its importance in functional dynamics in bull spermatozoa Molecular characterization of voltage-gated potassium channel (Kv) and its importance in functional dynamics in bull spermatozoa](/preview/png/8427100.png)
چکیده انگلیسی
Present study was undertaken to characterize the voltage gated potassium channel (Kv 1.1) in bull spermatozoa using sixty four ejaculates collected from four Hariana bulls. Functional characterization was undertaken using a selective blocker of Kv channel, 4-Aminopyridine (4-AP) while molecular presence of Kv on bull spermatozoa by immunoblotting and indirect immunofluorescence. Three sets of 100â¯Î¼L diluted sperm samples namely-negative control (100â¯Î¼L of sperm dilution medium (SDM) containing 10â¯Ãâ¯106â¯cells), vehicle control (99â¯Î¼L of SDM containing 10â¯Ãâ¯106â¯cells, and DMSO- 1â¯â¯Î¼L) and 4-AP treatment group (99â¯Î¼L of SDM containing 10â¯Ãâ¯106â¯cells, and drug 1â¯Î¼L 4-AP) were used in the study. Immunoblotting identified a single band of 56â¯kDa corresponding to Kv1.1 in Hariana bull spermatozoa. Immunolocalization showed the positive immunoreactivity at head, middle piece and principal piece of the spermatozoa for Kv 1.1. Blocking of Kv using 4-AP resulted in significant (pâ¯<â¯0.05) reduction in sperm progressive motility, per cent capacitated spermatozoa (B-pattern) and acrosome reacted (AR-pattern) spermatozoa, while significant (Pâ¯<â¯0.05) increase in per cent swollen spermatozoa. Blocking of Kv channels resulted in significantly (Pâ¯<â¯0.05) increased percentage of spermatozoa with lower mitochondrial transmembrane potential. Computer assisted semen analysis (CASA) of motion and kinematic parameters in 4-AP treated spermatozoa indicated reduction in sperm motion parameters like LIN, STR, VSL and VAP and higher ALH, VCL, and BCF indicating hyperactivity of spermatozoa. Based on our findings, it may be concluded that voltage-gated potassium channel (Kv) are present on bull spermatozoa and these are associated with functional dynamics of spermatozoa. However, based on our limited study, it is not possible to deduce that how these channels are associated with induction of hyperactivity. Therefore, further studies are warranted to unravel the mechanistic signaling pathways involved in Kv-mediated alterations in functional dynamics of spermatozoa.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 114, 1 July 2018, Pages 229-236
Journal: Theriogenology - Volume 114, 1 July 2018, Pages 229-236
نویسندگان
Rishi Kumar Gupta, Dilip Kumar Swain, Vijay Singh, Mukul Anand, Soumen Choudhury, Sarvajeet Yadav, Atul Saxena, Satish Kumar Garg,