کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8434265 | 1546639 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acquisition of tumorigenic potential and therapeutic resistance in CD133+ subpopulation of prostate cancer cells exhibiting stem-cell like characteristics
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Sox2SRY-Box 2MYC Proto-OncogeneCancer initiating cellsCICsDTXC-X-C motif chemokine receptor 4SOX-9Prominin-1CSCOct4CD133CXCR4ALDH1BMP2RUNX2FACSc-Myc - c-mycaldehyde dehydrogenase 1 - آلدئید دی هیدروژناز 1Gene expression - بیان ژنDocetaxel - داکتاکسلfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسProstate cancer - سرطان پروستاتcancer stem cell - سلولهای بنیادی سرطانیCancer stem cells - سلولهای بنیادی سرطانیrunt related transcription factor 2 - عامل رونویسی مربوط به ریت 2Biomarkers - نشانگر زیستی یا بیومارکرTherapeutic target - هدف درمانیbone morphogenetic protein 2 - پروتئین مورفوژنیک استخوان 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Acquisition of tumorigenic potential and therapeutic resistance in CD133+ subpopulation of prostate cancer cells exhibiting stem-cell like characteristics Acquisition of tumorigenic potential and therapeutic resistance in CD133+ subpopulation of prostate cancer cells exhibiting stem-cell like characteristics](/preview/png/8434265.png)
چکیده انگلیسی
The role of CD133 (Prominin-1) as a cancer stem cell marker may be useful for therapeutic approaches and prognostication in prostate cancer patients. We investigated the stem-cell-related function and biological features of a subpopulation of CD133+ cells isolated from established primary human prostate cancer cell lines. The CD133+ cells sorted from human prostate cancer 22Rv1 exhibited high clonogenic and tumorigenic capabilities, sphere forming capacity and serially reinitiated transplantable tumors in NOD-SCID mice. Gene profiling analysis of CD133+ cells showed upregulation of markers of stem cell differentiation (CD44, Oct4, SOX9 and Nanog), epithelial-to-mesenchymal transition (c-myc and BMI1), osteoblastic differentiation (Runx2), and skeletal morphogenesis (BMP2), compared to side population of CD133- cells. These cells are highly malignant and resistant to γ-radiation and chemotherapeutic drug, docetaxel. Importantly, a docetaxel-resistant subclone was more enriched in CD133+ cells with significant increase in Runx2 expression, compared to CD133- cells. Furthermore, knockdown of Runx2 in these cells resulted in differential response to chemotherapy, sensitizing them to increased cell death. These results demonstrate therapy-resistant population with stem-like features are distinct subpopulation of malignant cells that resides within parental cell lines. The molecular signature of CD133+ cells may lead to identification of novel therapeutic targets and prognostic markers in the treatment of prostate cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 430, 28 August 2018, Pages 25-33
Journal: Cancer Letters - Volume 430, 28 August 2018, Pages 25-33
نویسندگان
Rajnee Kanwal, Sanjeev Shukla, Ethan Walker, Sanjay Gupta,