کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
8435128 1546657 2018 8 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
MicroRNA-195 desensitizes HCT116 human colon cancer cells to 5-fluorouracil
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موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
MicroRNA-195 desensitizes HCT116 human colon cancer cells to 5-fluorouracil
چکیده انگلیسی
Multidrug resistance is one major barrier to successful chemotherapy. Although several studies have attempted to overcome resistance of cancer cells to anti-cancer drugs, key determinants of resistance remain largely unknown. The objective of this study was to investigate whether microRNAs might play a role in the acquisition of resistance. Human colorectal cancer HCT-116 cell lines were transduced with a lentivirus library containing 578 precursor microRNAs (miRNAs) to establish cell lines resistant to 5-fluorouracil (5-FU). Specific miRNAs were identified from four different resistant clones and a miR-195-expressing resistant clone (HCT-116_lenti-miR-195) was further investigated. The HCT-116_lenti-miR-195 cells showed resistant phenotype. These cells grew faster after 5-FU treatment compared to control cells (HCT-116_lenti-control). Check point kinase 1 (CHK1) and G2 check point kinase WEE1 were found to be direct targets of miR-195. Downregulation of miR-195 sensitized HCT-116 cells after 5-FU treatment. Our results demonstrate that miR-195 can promote acquisition of drug resistance to 5-FU.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 412, 1 January 2018, Pages 264-271
نویسندگان
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