کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456288 | 1548571 | 2016 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A retrospective evaluation method for in vitro mammalian genotoxicity tests using cytotoxicity index transformation formulae
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کلمات کلیدی
RICCRelative increase in cell countIWGTRPDOECDRCCECVAMChromosomal aberration test - آزمون انحراف کروموزومیMicronucleus test - آزمون میکرونوکلئوسChromosomal aberration - انحراف کروموزومیDatabase - بانک اطلاعاتی، دادِگان یا دِیتابِیسPopulation doubling - جمعیت دو برابر می شودtest guideline - دستورالعمل آزمونOrganization for Economic Co-operation and Development - سازمان همکاری و توسعه اقتصادیCytotoxicity - سمیت سلولیGenotoxicity - سمیت ژنتیکیfalse positive - مثبت کاذبMicronucleus - میکروسکوپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Although in vitro chromosomal aberration tests and micronucleus tests have been widely used for genotoxicity evaluation, false-positive results have been reported under strong cytotoxic conditions. To reduce false-positive results, the new Organization for Economic Co-operation and Development (OECD) test guideline (TG) recommends the use of a new cytotoxicity index, relative increase in cell count or relative population doubling (RICC/RPD), instead of the traditionally used index, relative cell count (RCC). Although the use of the RICC/RPD may result in different outcomes and require re-evaluation of tested substances, it is impractical to re-evaluate all existing data. Therefore, we established a method to estimate test results from existing RCC data. First, we developed formulae to estimate RICC/RPD from RCC without cell counts by considering cell doubling time and experiment time. Next, the accuracy of the cytotoxicity index transformation formulae was verified by comparing estimated RICC/RPD and measured RICC/RPD for 3 major chemicals associated with false-positive genotoxicity test results: ethyl acrylate, eugenol and p-nitrophenol. Moreover, 25 compounds with false-positive in vitro chromosomal aberration (CA) test results were re-evaluated to establish a retrospective evaluation method based on derived estimated RICC/RPD values. The estimated RICC/RPD values were in good agreement with the measured RICC/RPD values for every concentration and chemical, and the estimated RICC suggested the possibility that 12 chemicals (48%) with previously judged false-positive results in fact had negative results. Our method enables transformation of RCC data into RICC/RPD values with a high degree of accuracy and will facilitate comprehensive retrospective evaluation of test results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 796, 15 January 2016, Pages 1-7
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 796, 15 January 2016, Pages 1-7
نویسندگان
Yurika Fujita, Toshio Kasamatsu, Naohiro Ikeda, Naohiro Nishiyama, Hiroshi Honda,