کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8460661 | 1548989 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A bioluminescent mouse model of proliferation to highlight early stages of pancreatic cancer: A suitable tool for preclinical studies
ترجمه فارسی عنوان
یک مدل موش بیومواسانت کننده تکثیر برای برجسته کردن مراحل اولیه سرطان لوزالمعده: یک ابزار مناسب برای مطالعات پیش از موعد
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کلمات کلیدی
KPCComparative pathologyPancreatic ductal adenocarcinoma - آدنوکارسینوم پانکراس داکتالPDAC یا pancreatic ductal adenocarcinoma - آدنوکارسینومای داکتال پانکراسProliferation - ترویجbioluminescence imaging - تصویربرداری بیولوژیکیMolecular imaging - تصویربرداری مولکولیBLI - داشته باشد،Pancreatic cancer - سرطان پانکراسpancreatic intraepithelial neoplasia - نئوپلاسی داخل پاتوسی پانکراسPanIN - پانین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
Transgenic mouse models designed to recapitulate genetic and pathologic aspects of cancer are useful to study early stages of disease as well as its progression. Among several, two of the most sophisticated models for pancreatic ductal adenocarcinoma (PDAC) are the LSL-KrasG12D/+;Pdx-1-Cre (KC) and LSL-KrasG12D/+;LSL-Trp53R172H/+;Pdx-1-Cre (KPC) mice, in which the Cre-recombinase regulated by a pancreas-specific promoter activates the expression of oncogenic Kras alone or in combination with a mutant p53, respectively. Non-invasive in vivo imaging offers a novel approach to preclinical studies introducing the possibility to investigate biological events in the spatio/temporal dimension. We recently developed a mouse model, MITO-Luc, engineered to express the luciferase reporter gene in cells undergoing active proliferation. In this model, proliferation events can be visualized non-invasively by bioluminescence imaging (BLI) in every body district in vivo. Here, we describe the development and characterization of MITO-Luc-KC- and -KPC mice. In these mice we have now the opportunity to follow PDAC evolution in the living animal in a time frame process. Moreover, by relating in vivo and ex vivo BLI and histopathological data we provide evidence that these mice could represents a suitable tool for pancreatic cancer preclinical studies. Our data also suggest that aberrant proliferation events take place early in pancreatic carcinogenesis, before tumour appearance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 207, September 2016, Pages 2-8
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 207, September 2016, Pages 2-8
نویسندگان
Luisa de Latouliere, Isabella Manni, Carla Iacobini, Giuseppe Pugliese, Gian Luca Grazi, Pasquale Perri, Paola Cappello, Franco Novelli, Stefano Menini, Giulia Piaggio,