کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8464344 | 1549439 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A two-step dilution tris-egg yolk extender containing Equex STM significantly improves sperm cryopreservation in the African wild dog (Lycaon pictus)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A two-step dilution tris-egg yolk extender containing Equex STM significantly improves sperm cryopreservation in the African wild dog (Lycaon pictus) A two-step dilution tris-egg yolk extender containing Equex STM significantly improves sperm cryopreservation in the African wild dog (Lycaon pictus)](/preview/png/8464344.png)
چکیده انگلیسی
Conservation management of endangered African wild dogs (AWD; Lycaon pictus) can benefit greatly from development of sperm freezing and artificial insemination. Previous freezing attempts yielded nearly 0% motile sperm within 2â¯h of thawing. In this study, two canine freezing protocols were tested: Protocol 1: a one-step dilution in TRIS-20% egg yolk containing 8% glycerol; and Protocol 2: a two-step dilution in TRIS-20% egg yolk containing a final extender concentration of 5% glycerol and 0.5% Equex STM, coupled with a TRIS-citrate-fructose thawing solution. Semen was collected by electroejaculation from nâ¯=â¯24 AWDs, of which eight ejaculates of sufficient quality (four good quality with initial sperm motility of 75.0â¯Â±â¯4.4% and four poor quality; showing rapid decrease in sperm motility to 3.3â¯Â±â¯3.3% prior to freezing) were frozen. For good quality samples, motility and sperm motility index persisted for up to 8â¯h for Protocol 2, and was higher between 2 and 6â¯h after thawing with a decrease from 4â¯h of incubation. Motility dropped to nearly 0% after 2â¯h incubation for Protocol 1. Viability was higher for Protocol 2 throughout the 8â¯h of incubation, with a decrease after 6â¯h, compared to 4â¯h for Protocol 1. Acrosome integrity was higher for Protocol 2 throughout post-thaw incubation, with a decrease after 2â¯h for both protocols. Protocols did not differ in normal sperm morphology or DNA integrity. Poor quality samples yielded similar results, except for acrosome integrity, which declined for Protocol 2. In conclusion, a two-step dilution in TRIS-egg yolk-glycerol extender containing Equex STM yields significantly improved post-thaw quality and longevity of AWD spermatozoa, making it suitable for sperm banking and artificial insemination initiatives.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 80, February 2018, Pages 18-25
Journal: Cryobiology - Volume 80, February 2018, Pages 18-25
نویسندگان
Femke Van den Berghe, Monique Christina Johanna Paris, Michael Brent Briggs, Wenche Kristin Farstad, Damien Boyd Bertrand Paul Paris,