کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8469727 | 1549658 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-200b downregulates Kruppel Like Factor 2 (KLF2) during acute hypoxia in human endothelial cells
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کلمات کلیدی
HIF-1VEGFAVEGFR2HUVECHIFKLF2KDRHIF-2Kruppel like factor 2Human umbilical vascular endothelial cells - سلول های اندوتلیال عروق انسانی انسانیVascular endothelial growth factor A - فاکتور رشد اندوتلیال عروقی AHypoxia Inducible Factor - فاکتور قابل تشخیص هیپوکسیMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAkinase insert domain receptor - کیناز گیرنده دامنه را وارد کنید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The role of microRNAs in controlling angiogenesis is recognized as a promising therapeutic target in both cancer and cardiovascular disorders. However, understanding a miRNA's pleiotropic effects on angiogenesis is a limiting factor for these types of therapeutic approaches. Using genome-wide next-generation sequencing, we examined the role of an antiangiogenic miRNA, miR-200b, in primary human endothelial cells. The results indicate that miR-200b has complex effects on hypoxia-induced angiogenesis in human endothelia and importantly, that many of the reported miR-200b effects using miRNA overexpression may not be representative of the physiological role of this miRNA. We also identified the antiangiogenic KLF2 gene as a novel target of miR-200b. Our studies indicate that the physiological changes in miR-200b levels during acute hypoxia may actually have a proangiogenic effect through Klf2 downregulation and subsequent stabilization of HIF-1 signaling. Moreover, we provide a viable approach for differentiating direct from indirect miRNA effects in order to untangle the complexity of individual miRNA networks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 96, Issue 8, December 2017, Pages 758-766
Journal: European Journal of Cell Biology - Volume 96, Issue 8, December 2017, Pages 758-766
نویسندگان
Rafal Bartoszewski, Marcin Serocki, Anna Janaszak-Jasiecka, Sylwia Bartoszewska, Kinga Kochan-Jamrozy, Arkadiusz Piotrowski, JarosÅaw Króliczewski, James F. Collawn,