کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8470508 | 1549998 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genetic evidence for involvement of membrane trafficking in the action of 5-fluorouracil
ترجمه فارسی عنوان
شواهد ژنتیکی برای دخالت قاچاق غشا در اثر 5-فلوروکورازیل
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کلمات کلیدی
5-FUGFP5-FCEMMYPdEdinburgh Minimal Medium5-fluorocytosine - 5-فلوروکیتوزینYES - بلهthymidylate synthase - سمیاتید تیمیدیلاتYeast extract-Peptone-Dextrose - عصاره مخمر-پپتون-دکستروز5-fluorouracil - فلوروراسیل-۵، فلوئورواوراسیلMembrane trafficking - قاچاق غشاءFission yeast - مخمر شکافتنgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
To identify novel genes that mediate cellular sensitivity and resistance to 5-fluorouracil (5-FU), we performed a genome-wide genetic screening to identify altered susceptibility to 5-FU by Schizosaccharomyces pombe haploid nonessential gene deletion library containing 3004 deletion mutants. We identified 50 hypersensitive and 12 resistant mutants to this drug. Mutants sensitive or resistant to 5-FU were classified into various categories based on their putative functions. The largest group of the genes whose disruption renders cells altered susceptibility to 5-FU is involved in nucleic acid metabolism, but to our surprise, the second largest group is involved in membrane trafficking. In addition, several other membrane traffic mutants examined including gdi1-i11, ypt3-i5, Îryh1, Îric1, and Îaps1 exhibited hypersensitivity to 5-FU. Furthermore, we found that 5-FU in low concentration that generally do not affect cell growth altered the localization of Syb1, a secretory vesicle SNARE synaptobrevin which is cycled between the plasma membrane and the endocytic pathway. Notably, 5-FU at such low concentration also significantly inhibited the secretion of acid phosphatase. Altogether, our findings revealed the first evidence that 5-FU influences membrane trafficking as the potential underlying mechanism of the drug action.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 93, August 2016, Pages 17-24
Journal: Fungal Genetics and Biology - Volume 93, August 2016, Pages 17-24
نویسندگان
Lingling Hu, Fan Yao, Yan Ma, Qiannan Liu, Si Chen, Tsutomu Hayafuji, Takayoshi Kuno, Yue Fang,