کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8474554 | 1550428 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional differentiation of RAMP2 and RAMP3 in their regulation of the vascular system
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کلمات کلیدی
LDPIAdrenomedullin (AM)CGRPGPCRICGCLRadrenomedullin - آدرنومدولینAngiogenesis - آنژیوژنزIndocyanine green - ایندوسیانین سبزLaser Doppler perfusion imaging - تصویربرداری پرفیوژن لیزر داپلرramp - رمپLymphatic drainage - زهکشی لنفاویKnockout mice - موش نابود شدهwild-type - نوع وحشیReceptor activity-modifying protein - پروتئین تغییر دهنده فعالیت گیرندهcalcitonin gene-related peptide - پپتید مرتبط با ژن کلسی تونینcalcitonin receptor-like receptor - گیرنده ی گیرنده کلسایتونینG protein-coupled receptor - گیرندههای جفتشونده با پروتئین جی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Adrenomedullin (AM) is a vasoactive peptide that possesses various bioactivities. AM receptors are dimers consisting of CLR with one of two accessory proteins, RAMP2 or RAMP3. The functional difference between CLR/RAMP2 and CLR/RAMP3 and the relationship between the two receptors remain unclear. To address these issues, we generated RAMP2 and RAMP3 knockout (â/â) mice and have been studying their physiological activities in the vascular system. AMâ/Â â and RAMP2â/Â â mice die in utero due to blood vessel abnormalities, which is indicative of their essential roles in vascular development. In contrast, RAMP3â/Â â mice were born normally without any major abnormalities. In adult RAMP3â/Â â mice, postnatal angiogenesis was normal, but lymphangiography using indocyanine green (ICG) showed delayed drainage of subcutaneous lymphatic vessels. Moreover, chyle transport by intestinal lymphatics was delayed in RAMP3â/Â â mice, which also showed more severe interstitial edema than wild-type mice in a tail lymphedema model, with characteristic dilatation of lymphatic capillaries and accumulation of inflammatory cells. In scratch-wound assays, migration of isolated RAMP3â/Â â lymphatic endothelial cells was delayed as compared to wild-type cells, and AM administration failed to enhance the re-endothelialization. The delay in re-endothelialization was due to a primary migration defect rather than a decrease in proliferation. These results suggest that RAMP3 regulates drainage through lymphatic vessels, and that the AM-RAMP3 system could be a novel therapeutic target for controlling postoperative lymphedema.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 77, December 2014, Pages 73-85
Journal: Journal of Molecular and Cellular Cardiology - Volume 77, December 2014, Pages 73-85
نویسندگان
Akihiro Yamauchi, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Kyoko Igarashi, Yuichi Toriyama, Megumu Tanaka, Tian Liu, Xian Xian, Akira Imai, Liuyu Zhai, Shinji Owa, Takuma Arai, Takayuki Shindo,