کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8474668 | 1550430 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cardiac mitochondrial proteome dynamics with heavy water reveals stable rate of mitochondrial protein synthesis in heart failure despite decline in mitochondrial oxidative capacity
ترجمه فارسی عنوان
پویایی پروتئین میتوکندری قلبی با آب سنگین میزان ثابت سنتز پروتئین میتوکندریال در نارسایی قلبی را علیرغم کاهش ظرفیت اکسیداتیو میتوکندری نشان می دهد.
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کلمات کلیدی
FCRFSRIFMCID2H2OLTQTACSSMMCADProteome dynamicsLC-MS/MS - LC-MS / MSheavy water - آب سنگینLeft ventricular - بطن چپcollisionally induced dissociation - به صورت تصادفی القا شده استLinear trap quadrupole - تله خطی quadrupoledeuterium - دوتریوم، هیدروژن سنگینelectron transport chain - زنجیره انتقال الکترونMedium chain acyl-CoA dehydrogenase - زنجیره متوسط آکیل- CoA dehydrogenasesubsarcolemmal mitochondria - میتوکندری subarcolemmalinterfibrillar mitochondria - میتوکندری بین فیبریلاسیونMitochondria - میتوکندریاCardiac failure - نارسایی قلبیfractional synthesis rate - نرخ سنتز کسریتیfractional catabolic rate - نرخ کاتابولیک جزءETc - و غیرهliquid chromatography tandem mass spectrometry - کروماتوگرافی مایع اسپکترومتری دو طرفه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
We recently developed a method to measure mitochondrial proteome dynamics with heavy water (2H2O)-based metabolic labeling and high resolution mass spectrometry. We reported the half-lives and synthesis rates of several proteins in the two cardiac mitochondrial subpopulations, subsarcolemmal and interfibrillar (SSM and IFM), in Sprague Dawley rats. In the present study, we tested the hypothesis that the mitochondrial protein synthesis rate is reduced in heart failure, with possible differential changes in SSM versus IFM. Six to seven week old male Sprague Dawley rats underwent transverse aortic constriction (TAC) and developed moderate heart failure after 22Â weeks. Heart failure and sham rats of the same age received heavy water (5% in drinking water) for up to 80Â days. Cardiac SSM and IFM were isolated from both groups and the proteins were separated by 1D gel electrophoresis. Heart failure reduced protein content and increased the turnover rate of several proteins involved in fatty acid oxidation, electron transport chain and ATP synthesis, while it decreased the turnover of other proteins, including pyruvate dehydrogenase subunit in IFM, but not in SSM. Because of these bidirectional changes, the average overall half-life of proteins was not altered by heart failure in both SSM and IFM. The kinetic measurements of individual mitochondrial proteins presented in this study may contribute to a better understanding of the mechanisms responsible for mitochondrial alterations in the failing heart.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 75, October 2014, Pages 88-97
Journal: Journal of Molecular and Cellular Cardiology - Volume 75, October 2014, Pages 88-97
نویسندگان
Kadambari Chandra Shekar, Ling Li, Erinne R. Dabkowski, Wenhong Xu, Rogerio Faustino Jr., Peter A. Hecker, Fabio A. Recchia, Rovshan G. Sadygov, Belinda Willard, Takhar Kasumov, William C. Stanley,