کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8475219 | 1550445 | 2013 | 43 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of δ and κ opioid receptors with adenosine A1 receptors mediates cardioprotection by remote ischemic preconditioning
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کلمات کلیدی
SPTGNTIDPCPXIPCRIPCNTI5′-guanidinonaltrindole8-Cyclopentyl-1,3-dipropylxanthine - 8-Cyclopentyl-1،3-dipropylxanthineischemic preconditioning - preconditioning ایسکمیکCardiomyocytes - قلب و عروقRemote ischemic preconditioning - پیش از موعد مقابله با ایسکمیک راه دورAdenosine receptors - گیرنده های آدنوزینOpioid receptors - گیرنده های اپیدمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Multiple initiatives are underway to harness the clinical benefits of remote ischemic preconditioning (rIPC) based on applying non-invasive, brief, intermittent limb ischemia/reperfusion using an external occluder. However, little is known about how rIPC induces protection in cardiomyocytes, particularly through G-protein coupled receptors. In these studies, we determined the role of opioid and adenosine receptors and their functional interactions in rIPC cardioprotection. In freshly isolated cardiomyocytes subjected to 45-min simulated ischemia followed by 60-min simulated reperfusion, we examined the ability of plasma dialysate (derived from blood obtained from rabbits remotely preconditioned by application of brief cycles of hind limb ischemia/reperfusion, rIPC dialysate) to protect cells against necrosis. rIPC dialysate and selective activation of either δ-opioid receptors or κ-opioid receptors significantly reduced the % of dead cells after simulated ischemia and simulated reperfusion. Inhibition of adenosine A1 receptors, but not adenosine A3 receptors, blocked the protection by rIPC dialysate, δ-opioid receptor and κ-opioid receptor activation. In HEK293 cells expressing either hemagglutinin A-tagged δ-opioid receptors or hemagglutinin A-tagged κ-opioid receptors, selective immunoprecipitation of adenosine A1 receptors pulled down both δ-opioid and κ-opioid receptors. This molecular association of adenosine A1 receptors with δ-opioid and κ-opioid receptors was confirmed by reverse pull-down assays. These findings strongly suggest that rIPC cardioprotection requires the activation of δ-opioid and κ-opioid receptors and relies on these receptors functionally interacting with adenosine A1 receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 60, July 2013, Pages 142-150
Journal: Journal of Molecular and Cellular Cardiology - Volume 60, July 2013, Pages 142-150
نویسندگان
Harinee Surendra, Roberto J. Diaz, Kordan Harvey, Michael Tropak, John Callahan, Alina Hinek, Taneya Hossain, Andrew Redington, Gregory J. Wilson,