Drosophila melanogaster p24 trafficking proteins have vital roles in development and reproduction

► Reducing individual Drosophila p24 proteins results in developmental lethality. ► Drosophila p24 proteins function in female fecundity and male fertility. ► Adult neural expression of LOJ, ECA or CHOp24 is required for egg deposition. ► LOJ expression in octopaminergic or peptidergic neurons rescues loj mutant oviposition defects.