کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8477186 | 1550886 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adrenal CYP11B1/2 expression in primary aldosteronism: Immunohistochemical analysis using novel monoclonal antibodies
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کلمات کلیدی
IHACYP17CYP11B1CYP11B2zona reticularisHsd3b17α-hydroxylase - 17α-هیدروکسیلازAldosterone-producing adenoma - آدنوما تولید آلدوسترونPrimary aldosteronism - آلدسترونیزم اولیهMonoclonal antibody - آنتی بادی مونوکلونالzona glomerulosa - آنها glomerulosaAdrenal cortex - قشر آدرنالidiopathic hyperaldosteronism - هیدالدسترونیز ایدیوپاتیکAPA - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Adrenal CYP11B1/2 expression in primary aldosteronism: Immunohistochemical analysis using novel monoclonal antibodies Adrenal CYP11B1/2 expression in primary aldosteronism: Immunohistochemical analysis using novel monoclonal antibodies](/preview/png/8477186.png)
چکیده انگلیسی
CYP11B1 and CYP11B2 play pivotal roles in adrenocorticosteroids synthesis. We performed semi-quantitative immunohistochemical analysis of these proteins in adrenals from patients with primary aldosteronism using novel monoclonal antibodies. Clusters of cortical cells positive for CYP11B2 were detected in the zona glomerulosa (ZG) of normal adrenal gland (NA), idiopathic hyperaldosteronism (IHA) and the adjacent adrenal of aldosterone-producing adenoma (APA). In APA, heterogenous immunolocalization of CYP11B2 and diffuse immunoreactivity of CYP11B1 were detected in tumor cells, respectively. The relative immunoreactivity of CYP11B2 in the ZG of adjacent adrenal of APA was significantly lower than that of NA, IHA and APA tumor cells, suggestive of suppressed aldosterone biosynthesis in these cells. These findings did indicate the regulatory mechanisms of aldosterone biosynthesis were different between normal/hyperplastic and neoplastic aldosterone-producing cells in human adrenals. CYP11B2 immunoreactivity in the ZG could also serve as a potential immunohistochemical marker differentiating morphologically hyperplastic ZG of IHA and APA adjacent adrenal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 392, Issues 1â2, 5 July 2014, Pages 73-79
Journal: Molecular and Cellular Endocrinology - Volume 392, Issues 1â2, 5 July 2014, Pages 73-79
نویسندگان
Yasuhiro Nakamura, Takashi Maekawa, Saulo J.A. Felizola, Fumitoshi Satoh, Xin Qi, Carolina Velarde-Miranda, Maria W. Plonczynski, Kazue Ise, Kumi Kikuchi, William E. Rainey, Elise P. Gomez-Sanchez, Celso E. Gomez-Sanchez, Hironobu Sasano,