Synergistic actions of insulin-sensitive and Sirt1-mediated pathways in the differentiation of mouse embryonic stem cells to osteoblast

► Insulin and resveratrol acts synergistically for osteoblast differentiation. ► The osteoblast differentiation is mediated through Sirt1-dependent pathway. ► Sirt1 decreases adipocyte formation. ► Phosporylated Akt stabilizes β-catenin in the nucleus.