کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8477881 | 1550934 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
cAMP stimulation of StAR expression and cholesterol metabolism is modulated by co-expression of labile suppressors of transcription and mRNA turnover
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کلمات کلیدی
CREBHDACscAMP responsive element bindingcytochrome P450 11A1ACTHhuman antigen RPME-1CBPSIK1CDK7P450sccSF-1AUF1cAMP - cAMPAdrenal - آدرنالsteroidogenic acute regulatory - استروئیدوژنیک حاد تنظیم کنندهchromatin immunoprecipitation - ایمن سازی کروماتینStar - ستارهSteroidogenic factor 1 - عامل استروئیدوژنیک 1Cholesterol metabolism - متابولیسم کلسترولSIK - متداولadrenocorticotropic hormone - هورمون adrenocorticotropichistone deacetylases - هیستون deacetylasesCREB binding protein - پروتئین اتصال CREBCcaat-enhancer-binding protein - پروتئین اتصال دهنده تقویت کننده CcaatHuR - چگونهCHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The steroidogenic acute regulatory (StAR) protein is generated in rodents from 1.6Â kb and 3.5Â kb mRNA formed by alternative polyadenylation. The zinc finger protein, TIS11B (also Znf36L1), is elevated by cAMP in adrenal cells in parallel with StAR mRNA. TIS11b selectively destabilizes the 3.5Â kb mRNA through AU-rich sequences at the end of the 3â²UTR. siRNA suppression shows that TIS11b surprisingly increases StAR protein and cholesterol metabolism. StAR transcription is directly activated by PKA phosphorylation. cAMP responsive element binding (CREB) protein 1 phosphorylation is a key step leading to recruitment of the co-activator, CREB binding protein (CBP). A second protein, CREB regulated transcription coactivator (TORC/CRTC), enhances this recruitment, but is inhibited by salt inducible kinase (SIK). Basal StAR transcription is constrained through this phosphorylation of TORC. PKA provides an alternative stimulation by phosphorylating SIK, which prevents TORC inactivation. PKA stimulation of StAR nuclear transcripts substantially precedes TORC recruitment to the StAR promoter, which may, therefore, mediate a later step in mRNA production. Inhibition of SIK by staurosporine elevates StAR transcription and TORC recruitment to maximum levels, but without CREB phosphorylation. TORC suppression by SIK evidently limits basal StAR transcription. Staurosporine and cAMP stimulate synergistically. SIK targets the phosphatase, PP2a (activation), and Type 2 histone de-acetylases (inhibition), which may each contribute to suppression. Staurosporine stimulation through SIK inhibition is repeated in cAMP stimulation of many steroidogenic genes regulated by steroidogenic factor 1 (SF-1) and CREB. TIS11b and SIK may combine to attenuate StAR expression when hormonal stimuli decline.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 336, Issues 1â2, 10 April 2011, Pages 53-62
Journal: Molecular and Cellular Endocrinology - Volume 336, Issues 1â2, 10 April 2011, Pages 53-62
نویسندگان
Colin R. Jefcoate, Jinwoo Lee, Nadia Cherradi, Hiroshi Takemori, Haichuan Duan,