کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8478972 | 1551262 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Astrocytic γ-aminobutyric acid (GABA) transporters mediate guanidinoacetate transport in rat brain
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کلمات کلیدی
GAATauTGFAPCrtGPAAGATDAPIECFGATaCSFPBS4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولAstrocyte - آستروسیتγ-aminobutyric acid - اسید γ-آمینوبوتیریکCho - برایTaurine transporter - تائورین حملChinese Hamster Ovary - تخمدان هامستر چینیsolute carrier - حامل رقیبGABA transporter - حمل کننده GABAinterstitial fluid - مایع بینابینیextracellular fluid - مایع خارج سلولیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریISF - نیروهای امنیتی عراقGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالcreatine - کراتینcreatine transporter - کراتین حمل کنندهGABA - گاباGAMT - گامGuanidinoacetate - گوانیدین اسیدها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Guanidinoacetate (GAA) is a biosynthetic precursor of creatine, which plays a critical role in homeostasis of high-energy phosphates in the brain, but cerebral accumulation of GAA leads to neurological complications, such as epilepsy and seizures. The purpose of the present study was to clarify the contribution of the γ-aminobutyric acid (GABA) transport systems to GAA transport in astrocytes by means of uptake studies in rat brain slices, primary astrocyte cultures and Chinese hamster ovary (CHO) cells expressing human GABA transporters (GATs). GAA uptake by rat brain slices was Na+- and Clâ-dependent, and GABA-sensitive. The inhibitory effect of GABA, a common substrate of GATs, on GAA uptake by the brain slices was similar to that of β-alanine, a selective substrate of GAT2/Slc6a13, GAT3/Slc6a11, and taurine transporter (TauT)/Slc6a6. Taurine, a high-affinity substrate of TauT/Slc6a6, exhibited a lesser inhibitory effect. In contrast, betaine, a substrate of betaine-GABA transporter 1 (BGT1)/Slc6a12, and creatine, a substrate of creatine transporter (CRT)/Slc6a8, had little inhibitory effect. A similar inhibition profile was observed in primary-cultured astrocytes. CHO cells expressing human GAT2/SLC6A13, GAT3/SLC6A11 and BGT1/SLC6A12 exhibited GAA transport, whereas CHO cells expressing GAT1/SLC6A1 did not. The Michaelis-Menten values in CHO cells expressing GAT2/SLC6A13 and GAT3/SLC6A11 were similar to those in primary-cultured astrocytes. Overall, our results suggest that astrocytic GAT2/Slc6a13 and GAT3/Slc6a11 play major roles in GAA uptake as regulatory mechanisms of GAA in rat brain, while TauT/Slc6a6, BGT1/Slc6a12, and CRT/Slc6a8 make relatively small contributions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 113, February 2018, Pages 1-7
Journal: Neurochemistry International - Volume 113, February 2018, Pages 1-7
نویسندگان
Masanori Tachikawa, Ayane Yashiki, Shin-ichi Akanuma, Haruka Matsukawa, Soichiro Ide, Masabumi Minami, Ken-ichi Hosoya,