کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8478999 | 1551263 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Under stressful conditions activation of the ionotropic P2X7 receptor differentially regulates GABA and glutamate release from nerve terminals of the rat cerebral cortex
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کلمات کلیدی
HEPESN-methyl-d-glucamineEGTANMDGBzATPdl-threo-β-benzyloxyaspartic acidDPMsNCXconnexinsGATA-438079TTXEAATsMTLE2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid - 2- [4- (2-hydroxyethyl) piperazin-1-yl] ethanesulfonic aciddl-TBOA - DL-TBOAadenosine 5′-triphosphate - آدنوزین 5'-تری فسفاتATP - آدنوزین تری فسفات یا ATPGABA release - آزادی GABAethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیست (2-aminoethylether) -N، N، N '، N'-tetraacetic acidγ-aminobutyric acid - اسید γ-آمینوبوتیریکglutamate release - انتشار گلوتاماتBAPTA-AM - بیایپیتیای-AMtetrodotoxin - تترو دوتوکسین disintegrations per minute - تخریب در دقیقهlong term potentiation - تقویت طولانی مدتLTP - تقویت طولانی مدت یا LTP Min - حداقلGABA transporter - حمل کننده GABAAmino acid transporters - حمل کننده اسید آمینهCNS - دستگاه عصبی مرکزیminutes - دقایقsodium/calcium exchanger - سدیم / کلسیم مبدلcentral nervous system - سیستم عصبی مرکزیcounts per minute - شمار در هر دقیقهmesial temporal lobe epilepsy - صرع لوب صرع مزیالVeratridine - واراترییدGABA - گاباGlu - گلوglutamate - گلوتاماتP2X7 receptor - گیرنده P2X7
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
γ-Aminobutyric acid (GABA) and glutamate (Glu) are the main inhibitory and excitatory neurotransmitters in the central nervous system (CNS), respectively. Fine tuning regulation of extracellular levels of these amino acids is essential for normal brain activity. Recently, we showed that neocortical nerve terminals from patients with epilepsy express higher amounts of the non-desensitizing ionotropic P2X7 receptor. Once activated by ATP released from neuronal cells, the P2X7 receptor unbalances GABAergic vs. glutamatergic neurotransmission by differentially interfering with GABA and Glu uptake. Here, we investigated if activation of the P2X7 receptor also affects [3H]GABA and [14C]Glu release measured synchronously from isolated nerve terminals (synaptosomes) of the rat cerebral cortex. Data show that activation of the P2X7 receptor consistently increases [14C]Glu over [3H]GABA release from cortical nerve terminals, but the GABA/Glu ratio depends on extracellular Ca2+ concentrations. While the P2X7-induced [3H]GABA release is operated by a Ca2+-dependent pathway when external Ca2+ is available, this mechanism shifts towards the reversal of the GAT1 transporter in low Ca2+ conditions. A different scenario is verified regarding [14C]Glu outflow triggered by the P2X7 receptor, since the amino acid seems to be consistently released through the recruitment of connexin-containing hemichannels upon P2X7 activation, both in the absence and in the presence of external Ca2+. Data from this study add valuable information suggesting that ATP, via P2X7 activation, not only interferes with the high-affinity uptake of GABA and Glu but actually favors the release of these amino acids through distinct molecular mechanisms amenable to differential therapeutic control.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 112, January 2018, Pages 81-95
Journal: Neurochemistry International - Volume 112, January 2018, Pages 81-95
نویسندگان
Aurora R. Barros-Barbosa, Ãngela Oliveira, M. Graça Lobo, J. Miguel Cordeiro, Paulo Correia-de-Sá,