کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8492208 | 1552423 | 2012 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro assessment of three fibrolytic enzyme preparations as potential feed additives in equine diets
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کلمات کلیدی
NDFomHTLWSCNSPUACADFomFibrolytic enzymes - آنزیم های فیبرولیتیکUronic acids - اسیدهای ارونیکDigestible energy - انرژی قابل هضمgross energy - انرژی ناخالصEnzyme activity - فعالیت آنزیمLucerne - لوسرنorganic matter - ماده آلیdry matter - ماده خشکwater soluble carbohydrate - محلول در آب کربوهیدراتApparent digestibility - هضم ظاهریcrude protein - پروتئین خامNon-starch polysaccharide - پلی ساکارید غیر نشاسته
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
A series of in vitro experiments were conducted to assess three fibrolytic enzyme preparations as potential feed additives in equine diets. The three fibrolytic enzyme preparations were a concentrated cellulase (E1), an acid cellulase (E2) and a concentrated xylanase (E3). The enzymes were evaluated on their ability to modify the cell wall fraction of high-temperature dried lucerne (HTL) under various experimental conditions including differences in temperature, pH, incubation period, substrate levels and particle size to enable selection of the enzyme preparation most effective in the hydrolysis of lucerne. Results showed enzyme activities (as measured by reducing sugar assays) to be greatest at 50 °C, pH 5 and over an incubation period of greater than 20 h. E1 exhibited the greatest effect on total monosaccharide release from the HTL compared to E2 and E3. Moreover, dry matter (DM) and total non-starch polysaccharide (TNSP) losses were also greater in HTL treated with E1 compared to E2 and E3. Therefore, since the cell wall fraction of HTL contained substantial amounts of cellulose, the enzyme with the highest cellulase activity (Enzyme 1) was most effective in hydrolysing the cell walls of HTL. Consequently, it would appear that the application of exogenous fibrolytic enzyme preparations to forages requires the chemical characterisation of the target forage to enable selection of enzymes that are (a) most suitable to degrade the cell wall components of the candidate forage and (b) effective under field conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Feed Science and Technology - Volume 171, Issues 2â4, 10 February 2012, Pages 192-204
Journal: Animal Feed Science and Technology - Volume 171, Issues 2â4, 10 February 2012, Pages 192-204
نویسندگان
Jo-Anne M.D. Murray, Catherine Dunnett, Meriel J.S. Moore-Colyer, Annette C. Longland,