کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8498856 | 1553602 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NK-lysin from Oreochromis niloticus improves antimicrobial defence against bacterial pathogens
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
NK-lysin, an effector of cytotoxic T cells and natural killer cells, is a potent antimicrobial peptide widely distributed in mammals. Homologues of NK-lysin have been discovered in several teleost species, but only several of their natural functions was recorded so far. Here we identified an NK-lysin from Nile tilapia (Oreochromis niloticus), On-NKL, and analysed its expression model and biological effects on pathogen infection. The open reading frame of On-NKL sequence spans 432 bp, codes for 143 amino acids and shares 27%-62% overall sequence identities with NK-lysin of other species. The deduced mature peptide of On-NKL possesses a saposin B domain and six well-conserved cysteine residues that essential for antimicrobial activity by forming three intrachain disulphide bonds. The results of qRT-PCR showed that On-NKL expression was observed in multiple tissues and head kidney leucocytes and nonspecific cytotoxic cells (NCCs) and is most abundant in gills. After bacterial challenge, On-NKL expression significantly varied in different tissues and NCCs. Following bacterial infection, On-NKL-overexpressing fish featured significantly lower pathogen loads in tissues than control fish. On-NKL-overexpressing fish also exhibited 33.3% relative percent survival compare with control groups. Findings suggested that On-NKL could be the potential effector of NCCs and act as immune-related gene that enhances antimicrobial defence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 72, January 2018, Pages 259-265
Journal: Fish & Shellfish Immunology - Volume 72, January 2018, Pages 259-265
نویسندگان
Yu Huang, Qi Zheng, Jinzhong Niu, Jufen Tang, Bei Wang, Emmanuel Delwin Abarike, Yishan Lu, Jia Cai, Jichang Jian,