کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8505995 | 1555621 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Developmental capacity of Ascaridia galli eggs is preserved after anaerobic storage in faeces
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Developmental capacity of Ascaridia galli eggs is preserved after anaerobic storage in faeces Developmental capacity of Ascaridia galli eggs is preserved after anaerobic storage in faeces](/preview/png/8505995.png)
چکیده انگلیسی
The reliability of the results of in vitro studies such as detection of anthelmintic resistance often depends on the ability of the parasite eggs to develop under laboratory conditions. The aim of this study was to assess the embryonation capability of the chicken roundworm Ascaridia galli eggs after storage under different conditions. Two storage media for parasite eggs were used; faeces or water. Eggs in petri dishes (90 dishes in total) containing faces or water media were first exposed either to aerobic or anaerobic conditions at different temperatures (4â¯Â°C /+O2, 4â¯Â°C /âO2, 25â¯Â°C/âO2) for a maximum of 72 days. Every second week, materials from petri dishes in triplicates were recovered and incubated aerobically for two weeks at 25â¯Â°C. After the incubation, 200-300 eggs from each petri shish (sampling unit) were counted and the number of embryonated eggs was determined. Data was analyzed in R (version 3.4.3) A logistic regression model with the probability of an egg to embryonate as dependent variable and conditions, storage medium and time points as fixed effects with quasibinomial distribution was run. Least-square means were calculated and pairwise comparisons were made with the fixed effect factors (condition, storage medium and time point). Eggs in faeces had a significantly (pâ¯Ëâ¯0.05) higher embryonation than those in water, irrespective of storage conditions. At 4â¯Â°C embryonation tended to decline over time under aerobic conditions irrespective of the storage medium, whereas it remained constant following storage at anaerobic conditions. In contrast, anaerobic storage at the 25â¯Â°C negatively affected egg development in both media, except for day 14 in faeces. Our major finding was that eggs in faeces under anaerobic conditions and at 4â¯Â°C retained the highest rate of development, with a minimum decline in their developmental capacity over time compared to cleaned eggs stored in water.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 255, 15 May 2018, Pages 38-42
Journal: Veterinary Parasitology - Volume 255, 15 May 2018, Pages 38-42
نویسندگان
B. Tarbiat, S. Rahimian, D.S. Jansson, P. Halvarsson, J. Höglund,