کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8506108 | 1555624 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of Gliricidia sepium leaves intake on larval establishment of Cooperia punctata in calves and bio-guided fractionation of bioactive molecules
ترجمه فارسی عنوان
اثر مصرف گلریکیدیا برگ سفید در ایجاد لارو کوپیرا پانکتاتا در گوساله ها و تجزیه بیولوژیک مولکول های فعال زیستی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
The aims of this study were: 1) to assess the anthelmintic effect of Gliricidia sepium on the establishment of C. punctata third-stage larvae (L3) in calves, and 2) to isolate and to elucidate an anti-exsheathment phytochemical from the plant offered during the trial. Twelve ¾ Holsteinâ¯Ãâ¯Zebu calves were divided in two experimental groups: control (T1) and treatment (T2) (nâ¯=â¯6). After adaptation, each calf was infected with an oral dose of 400 C. punctata L3/Kg LW. Basal diet consisted of Digitaria decumbens hay (6.27% CP) and commercial concentrate (12% CP). In addition, during the experimental period T2 received fresh G. sepium leaves (26.88% CP) ad libitum. On day 9 post-infection, three calves per treatment were randomly selected for slaughter, and worm counts were performed. Larval establishment rates obtained were 13.44â¯Â±â¯0.13% and 3.1â¯Â±â¯1.42% for T1 and T2, respectively (Pâ¯<â¯.05). The reduction of larval establishment was 76.9%. The total length of worms recovered from the animals was also affected by the intake of G. sepium (Pâ¯<â¯.05). Phytochemicals present in G. sepium leaves offered to calves were isolated through silica gel columns and elucidated through Magnetic Nuclear Resonance (1H and 13C). Bio-guided isolation procedures lead to the elucidation of Oxytroside (Kaempferol 3-O-rhamnopyranosyl-(1â¯ââ¯6)-β-D-glucopyranoside-7-O-rhamnopyranoside), which fully inhibited the C. punctata exsheathment process (2400â¯Î¼gâ¯mLâ1). Gliricidia sepium represents an alternative to prevent severe C. punctata infections by reducing larval establishment in cattle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 252, 15 March 2018, Pages 137-141
Journal: Veterinary Parasitology - Volume 252, 15 March 2018, Pages 137-141
نویسندگان
von Son-de Fernex Elke, Alonso-DÃaz Miguel Ángel, Valles-de la Mora Braulio, Mendoza-de Gives Pedro, Castillo-Gallegos Epigmenio, Zamilpa Alejandro, González-Cortazar Manases,