کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8533296 | 1560459 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Amiodarone reduces depolarization-evoked glutamate release from hippocampual synaptosomes
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کلمات کلیدی
HEPES buffer mediumGDHGF109203XHBMH89CGP37157KT5720mEPSCsFura-2-AM - 2-فورا-AM6-Cyano-7-nitroquinoxaline-2,3-dione - 6-Cyano-7-nitroquinoxaline-2،3-dioneBSA - BSAd-AP5 - D-AP5DiSC3(5) - DiSC3 (5)dl-TBOA - DL-TBOA[Ca2+]c - [Ca2 +] cbovine serum albumin - آلبومین سرم گاوAmiodarone - آمیودارونglutamate release - انتشار گلوتاماتbisindolylmaleimide I - بیستینولیل مولارین Iminiature excitatory postsynaptic currents - جریانهای پسینپتیک هیجان انگیز مینیاتوریCNS - دستگاه عصبی مرکزیCNQX - سیانکیوایکسcentral nervous system - سیستم عصبی مرکزیSynaptosomes - سیناپتوزوم هاcytosolic free Ca2+ concentration - غلظت Ca2 + آزاد بدون سیتوزولFura-2-acetoxymethyl ester - فورا 2-اتوکسی متیل استرHippocampus - هیپوکامپ Ca2+ channels - کانال های Ca2 +glutamate dehydrogenase - گلوتامات دهیدروژناز
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Decreased brain glutamate level has emerged as a new therapeutic approach for epilepsy. This study investigated the effect and mechanism of amiodarone, an anti-arrhythmic drug with antiepileptic activity, on glutamate release in the rat hippocampus. In a synaptosomal preparation, amiodarone reduced 4-aminopyridine-evoked Ca2+-dependent glutamate release and cytosolic Ca2+ concentration elevation. Amiodarone did not affect the 4-aminopyridine-evoked depolarization of the synaptosomal membrane potential or the Na+ channel activator veratridine-evoked glutamate release, indicating that the amiodarone-mediated inhibition of glutamate release is not caused by a decrease in synaptosomal excitability. The inhibitory effect of amiodarone on 4-aminopyridine-evoked glutamate release was markedly decreased in synaptosomes pretreated with the Cav2.2 (N-type) and Cav2.1 (P/Q-type) channel blocker Ï-conotoxin MVIIC, the calmodulin antagonists W7 and calmidazolium, or the protein kinase A inhibitors H89 and KT5720. However, the intracellular Ca2+-release inhibitors dantrolene and CGP37157 had no effect on the amiodarone-mediated inhibition of glutamate release. Furthermore, amiodarone reduced the frequency of miniature excitatory postsynaptic currents without affecting their amplitude in hippocampal slices. Our data suggest that amiodarone reduces Ca2+ influx through N- and P/Q-type Ca2+ channels, subsequently reducing the Ca2+-calmodulin/protein kinase A cascade to inhibit the evoked glutamate release from rat hippocampal nerve terminals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmacological Sciences - Volume 133, Issue 3, March 2017, Pages 168-175
Journal: Journal of Pharmacological Sciences - Volume 133, Issue 3, March 2017, Pages 168-175
نویسندگان
Chia Yu Chang, Chi Feng Hung, Shu Kuei Huang, Jinn Rung Kuo, Su Jane Wang,