کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8538561 | 1561114 | 2018 | 43 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of nanocarrier potentiation on drug induced phospholipidosis in cultured cells and primary hepatocyte spheroids by high content imaging and analysis
ترجمه فارسی عنوان
تشخیص پتانسیل نانوذرات بر روی فسفولیپیدوز ناشی از دارو در سلول های کشت شده و اسپروئید های اولیه هپاتوسیت با استفاده از تصویربرداری و تحلیل محتوای بالا
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کلمات کلیدی
NBD-PENanocarrierLOPACDIPPLDAFMCLSMSpheroid - اسفروئیدind - اندرGraphene oxide - اکسید گرافنTem - این استInvestigational New Drug - داروهای جدید تحقیقاتیcationic amphiphilic drug - داروی amphiphilic کاتیونیCAD - طراحی به کمک رایانه یا کَدphospholipidosis - فسفولیپیدوزConfocal laser scanning microscope - میکروسکوپ اسکن لیزر ConfocalTransmission electron microscope - میکروسکوپ الکترونی انتقالAtomic Force Microscope - میکروسکوپ نیروی اتمیNanoparticle - نانوذارتHigh content screening - نمایش محتویات بالاPrimary hepatocyte - هپاتوسیت اولیه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Considerable effort has been made to develop nanocarriers for controlled drug delivery over the last decade, while it remains unclear how the strength of adverse drug effect will be altered when a drug is loaded on the nanocarrier. Drug-induced phospholipidosis (DIP) is characterized with excessive accumulation of phospholipids in cells and is common for cationic amphiphilic drugs (CAD). Previously, we have reported that PEGylated graphene oxide (PEG-GO) loaded with several CAD can potentiate DIP. In current study, we extended our study on newly identified phospholipidosis (PLD) inducers that had been identified from the Library of Pharmacologically Active Compounds (LOPAC), to investigate if PEO-GO loaded with these CAD can alter DIP. Twenty-two CAD were respectively loaded on PEG-GO and incubated with RAW264.7, a macrophage cell line. The results showed that when a CAD was loaded on PEG-GO, its strength of PLD induction can be enhanced, unchanged or attenuated. PEG-GO loaded with Ifenprodil exhibited the highest PEG-GO potentiation effect compared to Ifenprodil treatment alone in RAW264.7 cells, and this effect was confirmed in human hepatocellular carcinoma HepG2, another cell line model for PLD induction. Primary hepatocyte culture and spheroids mimicking in vivo conditions were used to further validate nanocarrier potentiation on DIP by Ifenprodil. Stronger phospholipid accumulation was found in PEG-GO/Ifenprodil treated hepatocytes or spheroids than Ifenprodil treatment alone. Therefore, evidences were provided by us that nanocarriers may increase the adverse drug effects and guidance by regulatory agencies need to be drafted for the safe use of nanotechnology in drug delivery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 348, 1 June 2018, Pages 54-66
Journal: Toxicology and Applied Pharmacology - Volume 348, 1 June 2018, Pages 54-66
نویسندگان
Xihui Zhang, Liecheng Yang, Yongming Liu, Zhentao Song, Jian Zhao, Dandan Chen, Huan Yu, Ruibin Li, Yangyun Wang, Kai Yang, Yu Chen, Menghang Xia, Leshuai W. Zhang,