کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8546029 | 1561687 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chronic exposure to a glyphosate-containing pesticide leads to mitochondrial dysfunction and increased reactive oxygen species production in Caenorhabditis elegans
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کلمات کلیدی
GFPHydroxyphenyl fluoresceinMitochondrial inhibitionCaenorhabditis Genetics CenterTMRENGMhpfC. elegansE. coliDMSO - DMSOCGC - GTCROS - ROSHydrogen peroxide - آب اکسیژنهtetramethylrhodamine ethyl ester - اتیل استر استرهای تترامتیل رودامینEscherichia coli - اشریشیا کُلیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancedihydroethidium - دی هیدروتیدیمDimethyl sulfoxide - دیمتیل سولفواکسیدTouchdown - زمین لرزهHerbicide - علف کشDHE - وgreen fluorescent protein - پروتئین فلورسنت سبزCaenorhabditis elegans - کرم الگانس Glyphosate - گلیفوزاتReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Glyphosate-containing herbicides are among the most widely-used in the world. Although glyphosate itself is relatively non-toxic, growing evidence suggests that commercial herbicide formulations may lead to increased oxidative stress and mitochondrial inhibition. In order to assess these mechanisms in vivo, we chronically (24Â h) exposed Caenorhabditis elegans to various concentrations of the glyphosate-containing herbicide TouchDown (TD). Following TD exposure, we evaluated the function of specific mitochondrial electron transport chain complexes. Initial oxygen consumption studies demonstrated inhibition in mid- and high-TD concentration treatment groups compared to controls. Results from tetramethylrhodamine ethyl ester and ATP assays indicated reductions in the proton gradient and ATP levels, respectively. Additional studies were designed to determine whether TD exposure resulted in increased reactive oxygen species (ROS) production. Data from hydrogen peroxide, but not superoxide or hydroxyl radical, assays showed statistically significant increases in this specific ROS. Taken together, these data indicate that exposure of Caenorhabditis elegans to TD leads to mitochondrial inhibition and hydrogen peroxide production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 57, January 2018, Pages 46-52
Journal: Environmental Toxicology and Pharmacology - Volume 57, January 2018, Pages 46-52
نویسندگان
Denise C. Bailey, Callie E. Todt, Shelbie L. Burchfield, Aireal S. Pressley, Rachel D. Denney, Isaac B. Snapp, Rekek Negga, Wendy L. Traynor, Vanessa A. Fitsanakis,