کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8550504 1562037 2016 30 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alterations in mRNA and protein expression of glutamate transporters in rat hippocampus after paraoxon exposure
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Alterations in mRNA and protein expression of glutamate transporters in rat hippocampus after paraoxon exposure
چکیده انگلیسی
Organophosphates affect brain function through a variety of mechanisms beyond their shared role as cholinesterase inhibitors. The aim of the current study was to investigate the changes in the expression of glial (GLAST and GLT-1) and neuronal (EAAC1) glutamate transporters at mRNA and protein levels in paraoxon-treated rat hippocampus. Adult male Wistar rats were intraperitoneally treated with either vehicle (corn oil) or one of three dosages of paraoxon (0.3, 0.7 or 1 mg/kg). After 4 or 18 h, both hippocampi of each rat were collected to detect mRNA and protein expression of glutamate transporters using the quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting, respectively. Animals treated with 0.3 mg/kg paraoxon showed no difference in mRNA and protein levels of the glutamate transporters when compared with control group. At 4 h after exposure with 0.7 and 1 mg/kg paraoxon, the expression of GLAST and GLT-1 increased at mRNA and protein levels and remained elevated after 18 h. No difference in the expression of EAAC1 at mRNA and protein levels was observed in any paraoxon-treated groups compared with the control group. This study showed an increased expression of glial (GLAST and GLT-1), but not neuronal (EAAC1) glutamate transporters, in adult rat hippocampus following administration of convulsive dosages of paraoxon. These suggest a protective and compensatory adaptation for effective uptake of glutamate in hippocampus induced by paraoxon and thus attenuating seizure activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: NeuroToxicology - Volume 57, December 2016, Pages 251-257
نویسندگان
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