کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8624058 | 1567909 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High expression and localization of β-catenin and epidermal growth factor receptor identify high risk papillary thyroid carcinoma patients
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High expression and localization of β-catenin and epidermal growth factor receptor identify high risk papillary thyroid carcinoma patients High expression and localization of β-catenin and epidermal growth factor receptor identify high risk papillary thyroid carcinoma patients](/preview/png/8624058.png)
چکیده انگلیسی
We have evaluated the clinical significance of deregulated expression of β-catenin and epidermal growth factor receptor (EGFR) during papillary thyroid carcinoma (PTC) progression. Immunohistochemical expression of β-catenin and EGFR was analyzed in 104 archival tissues of PTC and 19 matched lymph node metastases (LNMs). β-catenin (39/104, 37.5%) and EGFR (58/104, 55.7%) were co-expressed and co-localized in primary PTCs (pâ¯<â¯.0001), which was confirmed by double immunofluorescent staining. The high expression of each molecule, as well as their high cytosolic co-expression, correlated with adverse clinicopathological features of the patients (pâ¯<â¯.05). High expression of the proteins did not associate with the presence of BRAFV600E mutation (pâ¯>â¯.05), tested by mutant allele-specific PCR amplification. Although nuclear localization of β-catenin was found in a subset of PTC patients (16/104, 15.4%), no β-catenin mutations were found in exon 3 of the CTNNB1 gene (screened by PCR in combination with denaturing gradient gel electrophoresis and confirmed by next generation sequencing). Cases with additional nuclear β-catenin staining showed strong association with high EGFR expression (15/16, 93.7%), the presence of capsule invasion (12/16, 81.25%) and regional LNM (9/16, 52.3%). In corresponding LNMs, β-catenin and EGFR expressions were maintained at high levels or further increased. Co-expression of high levels of β-catenin and EGFR in association with clinicopathological features implicates their clinical utility in risk stratification of PTC patients, and supports the possibility of crosstalk between Wnt/β-catenin and EGFR signaling during PTC progression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 105, Issue 2, October 2018, Pages 181-189
Journal: Experimental and Molecular Pathology - Volume 105, Issue 2, October 2018, Pages 181-189
نویسندگان
Tijana IÅ¡iÄ DenÄiÄ, Aleksandar Bartolome, Sonja Å elemetjev, Ilona ÄoriÄ, Svetislav TatiÄ, Vladan ŽivaljeviÄ, Dubravka CvejiÄ,