کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8630884 | 1568844 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Distribution and morphology of gonadotropin-releasing hormone neurons in the hypothalamus of an induced ovulator - The llama (Lama glama)
ترجمه فارسی عنوان
توزیع و مورفولوژی نورون های هورمون آزاد کننده گنادوتروپین در هیپوتالاموس تخمک گذاری شده توسط تخمک گذاری - لاما (لما گلما)
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کلمات کلیدی
PBSOIFGnRHBSA - BSAbovine serum albumin - آلبومین سرم گاوOvulation-inducing factor - عامل القاء تخمک گذاریphosphate buffer saline - فسفات بافر شورNeurons - نورون ها،یاخته های عصبیGonadotropin-releasing hormone - هورمون آزاد کننده گنادوتروپینgonadotrophin-releasing hormone - هورمون آزاد کننده گنادوتروپینluteinizing hormone - هورمون جسم زردHypothalamus - هیپوتالاموس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
چکیده انگلیسی
Gonadotropin-releasing hormone (GnRH) is a decapeptide involved in the regulation of reproduction in all mammals, but the distribution of GnRH neurons within the brain varies widely among species. The objective of the present study was to characterize the number and distribution of GnRH neurons in the hypothalamus and preoptic area of llamas, an induced ovulator. The brains of female llamas (nâ¯=â¯4) were fixed, frozen and sectioned serially every 50 µm in the transverse (coronal) plane. Every 10th section was stained for immunohistochemical detection of GnRH-positive neuron cell bodies and fibers by incubation with 3,3â²-diaminobenzidine. The number of counted immunoreactive cells ranged from 222 to 250 (â241â¯Â±â¯13 cells in the preoptic area and hypothalamus per animal) and were localized in the medio-basal hypothalamus (44.3%), anterior hypothalamus (27%), preoptic area (14.9%), diagonal band of Broca/medial septum (13.4%), and mammillary area (0.5%). The immunoreactive cells were not localized in specific hypothalamic nuclei, but rather appeared to be distributed diffusely. The highest concentration of immunoreactive neuron fibers was in the median eminence (Pâ¯<â¯0.05), but fibers were identified in most of the areas analyzed, including the neurohypophysis. The GnRH neurons within the hypothalamus displayed monopolar (33%), bipolar (39%), and multipolar (28%) morphologies. The bipolar type was most common in the medio-basal region (40%; Pâ¯<â¯0.05). We conclude that GnRH neurons and fibers form a network within the anterior and medio-basal hypothalamus of llamas, suggesting the central location of mechanisms controlling reproductive processes in llamas (i.e., induced ovulation).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 263, 1 July 2018, Pages 43-50
Journal: General and Comparative Endocrinology - Volume 263, 1 July 2018, Pages 43-50
نویسندگان
Rodrigo A. Carrasco, Jaswant Singh, Gregg P. Adams,