کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8631110 | 1568848 | 2018 | 49 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of estrogen receptors in female rainbow trout (Oncorhynchus mykiss) during first ovarian development and under dense rearing condition
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کلمات کلیدی
GSIqPCRHSIOncorhynchus mykissSD1enzyme-linked immuno sorbent assay - آنزیم وابسته به ایمنی جذب آزمونEstradiol - استرادیولELISA - تست الیزاreal-time quantitative PCR - زمان واقعی PCR کمیRainbow trout - قزل آلای رنگین کمانLipid droplets - قطرات لیپیدZona pellucida - منطقه کوچکNucleolus - هستکestrogen receptors - گیرنده های استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To study the expression of four estrogen receptor genes (erα1, erα2, erβ1, erβ2) of female rainbow trout (Oncorhynchus mykiss) during first ovarian development, trouts were sampled from different ovarian stages. Serum E2 (estradiol) was measured by ELISA and estrogen receptors mRNA expression were examined by qRT-PCR. Our results showed a close association between increased erα1 and vitellogenin mRNA expression during ovarian maturation and increased erα2 mRNA expression in mature ovarian stages. Correlation analysis revealed that a negative relationship between serum E2 and ovarian erβ1 (or hepatic erβ2), but ovarian erβ2 mRNA expression was relatively unchanged during first ovarian development. Trout were also reared in different densities as stocking density 1, 2 and 3 (SD1, 4.6-31.1â¯kg/m3; SD2, 6.6-40.6â¯kg/m3; SD3, 8.6-49.3â¯kg/m3) to elucidate effects of high density on estrogen receptor expression. Histology observation showed ovarian development of trout in higher densities were retard with a relatively early stage and fewer vitellogenin accumulation. Trout in high densities showed significantly decreased serum E2, erα mRNA expression and increasing trends of erβ mRNA expression. A noticeable increase of ovarian erβ2 mRNA expression was seen in trout when density is approaching to 50â¯kg/m3. In conclusion, we may hypothesize that increased erβ mRNA expression triggered by high density result in decreased erα mRNA expression and vitellogenesis. As a result, ovarian development in higher densities was retard.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 259, 1 April 2018, Pages 1-11
Journal: General and Comparative Endocrinology - Volume 259, 1 April 2018, Pages 1-11
نویسندگان
Zhi-Shuai Hou, Hai-Shen Wen, Ji-Fang Li, Feng He, Yun Li, Ya-Xiong Tao,