کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8631176 | 1568848 | 2018 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Temperature- vs. estrogen-induced sex determination in Caiman latirostris embryos: Both females, but with different expression patterns of key molecules involved in ovarian development
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ERαESRSPCNATAp63p63ERβTSDIODGAMDABTSP17β-estradiol - 17β استرادیولEDCs - EDC هاaromatase - آروماتازProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیIHC - ایمونوهیستوشیمیImmunohistochemistry. - ایمونوهیستوشیمیintegrated optical density - تراکم نوری یکپارچهEndocrine-disrupting compounds - ترکیبات غشایی ناپایدارTemperature-dependent sex determination - تعیین جنسیت وابسته به دماSex reversal - تغییر جنسیتTUNEL - تونلApoptosis - خزان یاختهایReptile - خزندهdiaminobenzidine - دیامینو بنزیدینVasa - شماCaiman latirostris - کیمان لاتروستریسEstrogen receptor - گیرنده استروژنEstrogen receptor alpha - گیرنده استروژن آلفاEstrogen receptor beta - گیرنده استروژن بتاSteroid hormone receptors - گیرنده های هورمون استروئیدProgesterone receptor - گیرنده پروژسترون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Caiman latirostris is a species with temperature dependent sex determination (TSD), which implies that the incubation temperature of the eggs is the main factor that determines the sex during a thermo-sensitive period (TSP). However, estrogens play a critical role in this process. The administration of 17β-estradiol (E2) previous to TSP overrides the effects of male incubation temperature, producing phenotypic females. This effect has been defined as sex reversal or estrogen-induced sex determination (E2SD). The aim of the present study is to describe similarities and differences in the effects of TSD and E2SD treatment conditions on ovary development. Our results show that the two treatment conditions studied are able to produce different ovaries. Treatment with E2 modified the expression pattern of estrogen receptor alpha and progesterone receptor, and expression of the enzyme aromatase. Moreover, in E2SD females, the proliferation/apoptosis dynamic was also altered and high expression of TAp63 was observed suggesting the presence of greater DNA damage in germ cells. To the best of our knowledge, this is the first report that describes the morphology of the female gonad of C. latirostris in three stages of embryonic development and shows the expression of TAp63 during the gonad development of a reptile. It is important to emphasize that the changes demonstrated in E2SD female gonads of embryos show that environmental compounds with proven estrogenic activity alter the follicular dynamics of C. latirostris in neonatal as much as in juvenile animals, endangering their reproductive health and possibly bringing consequences to ecology and evolution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 259, 1 April 2018, Pages 176-188
Journal: General and Comparative Endocrinology - Volume 259, 1 April 2018, Pages 176-188
نویسندگان
Guillermina Canesini, Cora Stoker, Germán H. Galoppo, Milena L. Durando, MarÃa V. Tschopp, Enrique H. Luque, Mónica M. Muñoz-de-Toro, Jorge G. Ramos,