کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8634058 | 1569090 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toll-like receptor signaling and serum levels of interferon β and lipopolysaccharide binding protein are related to abdominal obesity: a case-control study between metabolically healthy and metabolically unhealthy obese individuals
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
It is still unclear whether toll-like receptor (TLR) signaling and serum levels of inflammatory markers in metabolically unhealthy abdominally obese (MUAO) are due to their obesity and/or their metabolic state. We hypothesized that abdominal obesity is an important mediator of the association of metabolic state with TLR signaling and serum inflammatory markers. Therefore, in this case-control study, we compared the expression levels of TLR4 and Toll/interleukin-1 receptor domain containing adaptor protein-inducing interferon β (TRIF) and serum concentrations of interferon β and lipoprotein-binding protein (LBP) in metabolically healthy abdominally obese (MHAO) and MUAO individuals. Basal blood samples from 65 abdominally obese subjects with waist circumference (WC) of at least 95 cm were collected to determine serum metabolic parameters, IFNβ, and LBP. Those with 3 or more metabolic alterations were defined as MUAO (nâ¯=â¯34), and those having 2 or less were classified as MHAO (nâ¯=â¯31). Furthermore, messenger RNA (mRNA) was isolated from peripheral blood mononuclear cells. TLR4 and TRIF gene expression assay was performed using quantitative real-time polymerase chain reaction. There were significant differences in serum fasting blood sugar (Pâ¯=â¯.017), triglyceride (Pâ¯<â¯.001), cholesterol (Pâ¯=â¯.002), and low-density lipoprotein cholesterol (Pâ¯=â¯.034) between the MUAO and MHAO groups, whereas no significant difference was observed in the expression ratio of TLR4 and TRIF mRNA and serum levels of IFNβ and LBP. However, a significant correlation was noticed between mRNA expression levels of TLR4 and TRIF (râ¯=â¯0.50, Pâ¯<â¯.001) and serum IFNβ and LBP (râ¯=â¯0.70, Pâ¯<â¯.001). It is concluded that the expression levels of TLR4 and TRIF as well as serum IFNβ and LBP are more related to abdominal obesity than to metabolic health.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 55, July 2018, Pages 11-20
Journal: Nutrition Research - Volume 55, July 2018, Pages 11-20
نویسندگان
Mahsa Naghizadeh, Behzad Baradaran, Maryam Saghafi-Asl, Parichehr Amiri, Dariush Shanehbandi, Nahid Karamzad, Leila Mohamed-Khosroshahi,