کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645040 | 1569774 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The SlNAC8 gene of the halophyte Suaeda liaotungensis enhances drought and salt stress tolerance in transgenic Arabidopsis thaliana
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کلمات کلیدی
GFPPSIIERFDAPIDREB4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولDrought stress - تنش خشکیSalt stress - تنش شوریNAC transcription factor - فاکتور رونویسی NACPhotosystem II - فتوسیستم 2Myb - مبینmyeloblastosis - میلوللاستوزgreen fluorescent protein - پروتئین فلورسنت سبزTransgenic plants - گیاهان تراریخته
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The SlNAC8 gene of the halophyte Suaeda liaotungensis enhances drought and salt stress tolerance in transgenic Arabidopsis thaliana The SlNAC8 gene of the halophyte Suaeda liaotungensis enhances drought and salt stress tolerance in transgenic Arabidopsis thaliana](/preview/png/8645040.png)
چکیده انگلیسی
NAC (NAM, ATAF1/2 and CUC) transcription factors play an important role in resisting abiotic stress in plants. In this study, a novel NAC gene, designated SlNAC8 from Suaeda liaotungensis K. was characterized. SlNAC8 protein is localized in the nucleus, and the yeast one-hybrid screening showed that it contains an activation domain in its C-terminus and functions as a transcriptional activator. Gene expression analysis revealed that it is induced by drought and salt stress. Arabidopsis plants overexpressing SlNAC8 demonstrated enhanced tolerance to drought and salt stress, showing significant advantages in seed germination, root growth, shoot growth, and survival rate compared with controls. Moreover, transgenic plants had a significantly higher proline concentration, antioxidant enzyme activity (superoxide dismutase, peroxidase, and catalase), and level of chlorophyll fluorescence than wild-type, and a significantly lower malondialdehyde concentration and electrolyte leakage under drought and salt stress. The overexpression of SlNAC8 in transgenic plants also enhanced the expression of stress-responsive genes such as RD20, GSTF6, COR47, RD29A, RD29B, and NYC1. In summary, SlNAC8, as a transcription factor, may change the physiological-biochemical characteristic of plants by regulating the expression of stress-responsive genes and enhance the drought and salt stress tolerance of plants. SlNAC8 can be utilized for developing drought and salinity tolerance in crop plants through genetic engineering.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 662, 1 July 2018, Pages 10-20
Journal: Gene - Volume 662, 1 July 2018, Pages 10-20
نویسندگان
Dandan Wu, Yinghao Sun, Hongfei Wang, He Shi, Mingxing Su, Hongyan Shan, Tongtong Li, Qiuli Li,