کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645255 | 1569779 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
microRNA-520f inhibits hepatocellular carcinoma cell proliferation and invasion by targeting TM4SF1
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کلمات کلیدی
TM4SF1MMP-2/9FITCPVDFHRPRIPAqRT-PCR3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-سمتیلیتیازول-2-ییل) -2،5-دی فینیل تترازولیم برومیدDMSO - DMSOHCC - HCCMAPK - MAPKMTT - MTTsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدCell proliferation - تکثیر سلولیradioimmunoprecipitation assay - سنجش radioimmunoprecipitationfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استHorseradish peroxidase - پراکسیداز هوررادیشp38 mitogen-activated protein kinase - پروتئین کیناز متیوژن فعال p38Propidium iodide - پروتئین یدیدPolyvinylidene fluoride - پلی وینیلیدین فلورایدHepatocellular carcinoma - کارسینوم هپاتوسلولار(کارسینوم سلولهای استخوانی)
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
microRNAs (miRNAs) are reported to play crucial roles in tumorigenesis. Dysregulation of miR-520f has been implicated to be involved in several cancer progressions. However, the biological functions of miR520f in hepatocellular carcinoma (HCC) remain unclear. Thus, the molecular mechanism underlying miR-520f on HCC development was investigated in this study. Here, we found that miR-520f was remarkably down-regulated in human HCC samples and cell lines compared to paired normal tissues and cell lines as detected by qRT-PCR. Furthermore, the deregulated miR-520f was strongly associated with larger tumor size, advanced TNM stage, and metastasis in HCC patients. Functional investigations revealed that overexpression of miR-520f significantly suppressed cell proliferation, invasion and migration, caused cell cycle arrested at G0/G1 phase, and promoted cell apoptosis in HCC cells according to MTT, colony formation, transwell, and flow cytometry assays, respectively, whereas, downregulation of miR-520f exhibited inverse effects. Transmembrane-4 L-Six family member-1 (TM4SF1) was identified as a direct target of miR-520f, and an inverse relationship was found between miR-520f and TM4SF1 mRNA levels in HCC specimens. Rescue experiments suggested that restoration of TM4SF1 partially abolished miR-520f-meidated cell proliferation and invasion inhibition in HCC cells through regulating P13K/AKT and p38 MAPK signaling pathways. In conclusion, these data indicated that miR-520f acted as tumor suppressor in HCC proliferation and invasion by targeting TM4SF1, which might provide potential therapeutic evidence for HCC patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 657, 30 May 2018, Pages 30-38
Journal: Gene - Volume 657, 30 May 2018, Pages 30-38
نویسندگان
Xiaoqin Du, Wanhu Fan, Yunru Chen,