کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645288 | 1569780 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a tyrosinase gene and its functional analysis in melanin synthesis of Pteria penguin
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کلمات کلیدی
CREBPyrrole-2,3,5-tricarboxylic acidBcl-2PDCADHICAPTCAMITFPAX35,6-Dihydroxyindole - 5،6-دی هیدروکسی اندول5,6-dihydroxyindole-2-carboxylic acid - 5،6-دی هیدروکسی اندول-2-کربوکسیلیک اسیدLC-MS/MS - LC-MS / MSRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهdHI - بزTyr - تیرTyrosinase - تیروزیناز Microphthalmia-associated transcription factor - فاکتور رونویسی مرتبط با میکرو فتالمیB-cell lymphoma 2 - لنفوم سلول B 2melanin - ملانینUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهPteria penguin - پگنگین پتریا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Tyrosinase is a key rate-limiting enzyme in melanin synthesis. In this study, a new tyrosinase gene (Tyr) was identified from Pteria penguin and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of PpTyr was 1728â¯bp long, containing a 5â²untranslated region (UTR) of 11â¯bp, a 3â²UTR of 295â¯bp, and an open reading fragment of 1422â¯bp encoding 473 amino acids. Amino acid alignment showed PpTyr had the highest (50%) identity to tyrosinase-like protein 1 from Pinctada fucata. Phylogenetic tree analysis classified PpTyr into α-subclass of type-3 copper protein. Tissue expression analysis indicated that PpTyr was highly expressed in mantle, a nacre formation related tissue. After PpTyr RNA interference, PpTyr mRNA was significantly inhibited by 71.0% (Pâ¯<â¯0.05). For other melanin-related genes, PpCreb2 and PpPax3 expression showed no significant change, but PpBcl2 was obviously increased. By liquid chromatograph-tandem mass spectrometer (LC-MS/MS) analysis, the total content of PDCA (pyrrole-2, 3-dicarboxylic acid) and PTCA (pyrrole-2,3,5-tricarboxylic acid), as main markers for eumelanin, was sharply decreased by 66.6% after PpTyr RNAi (Pâ¯<â¯0.05). The percentage of PDCA was also obviously decreased from 20.1% to 13.9%. This indicated that tyrosinase played a key role in melanin synthesis and color formation of P. penguin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 656, 20 May 2018, Pages 1-8
Journal: Gene - Volume 656, 20 May 2018, Pages 1-8
نویسندگان
Feifei Yu, Zhenni Pan, Bingliang Qu, Xiangyong Yu, Kaihang Xu, Yuewen Deng, Feilong Liang,