کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645594 | 1569789 | 2018 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression profiles and functional annotation analysis of mRNAs in suprachiasmatic nucleus of Clock mutant mice
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کلمات کلیدی
SCNVIPR2NPY1RHoxA5GABRA2GATA binding protein 4GATA4MT2MT1vasopressin 1a receptorAVPR1aTLRqRT-PCRVIPDEGsAVPbipolar disorder - اختلال دو قطبیMolecular function - تابع مولکولیMicroarray - ریزآرایهReal time quantitative PCR - زمان واقعی PCR کمیCircadian Clock - ساعت دیجیتالFca - فاکسBiological processes - فرآیندهای بیولوژیکیwild type - نوع وحشیSuprachiasmatic nucleus - هستههای سوپراکیاسماتیکvasopressin - وازوپرسینvasoactive intestinal peptide - پپتید روده روده ایolfactory bulb - پیاز بویاییDifferentially expressed genes - ژن های متفاوت بیان شده استToll-like receptors - گیرنده های پولی مانند
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The core circadian clock gene, Clock, is a positive component of the transcription/translation feedback loop in the master pacemaker suprachiasmatic nucleus (SCN) in mammals. The robust daytime peak of some clock genes in the wild-type SCN is absent in Clock mutant mice. However, very little is known about the impact of Clock mutation on the expression of other functional genes in SCN. Here, we performed cDNA microarray and found 799 differentially expressed genes (DEGs) at zeitgeber time 2 (ZT2) and 1289 DEGs at ZT14 in SCN of Clockâ³19/â³19 mutant mice. KEGG pathway analysis showed that the changed mRNAs were highly associated with hedgehog signaling pathway, retinol metabolism, allograft rejection, drug metabolism, hematopoietic cell lineage and neuroactive ligand-receptor interaction. The top 14 and 71 hub genes were identified from the protein-protein interaction (PPI) network at ZT2 and ZT14, respectively. The sub-networks revealed hub genes were involved in olfactory transduction and neuroactive ligand-receptor interaction pathways. These results demonstrate the Clockâ³19/â³19 mutation alters the expression of various genes involved in a wide spectrum of biological function in mouse SCN, which are helpful for better understanding the function of Clock and potential regulatory mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 647, 20 March 2018, Pages 107-114
Journal: Gene - Volume 647, 20 March 2018, Pages 107-114
نویسندگان
Yanli Wang, Ke Lv, Mei Zhao, Fengji Liang, Hailong Chen, Guohua Ji, Tingmei Wang, Yongliang Zhang, Hongqing Cao, Yinghui Li, Lina Qu,