کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8647011 | 1570146 | 2018 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel HBV recombinants between genotypes B and C in 3â²-terminal reverse transcriptase (RT) sequences are associated with enhanced viral DNA load, higher RT point mutation rates and place of birth among Chinese patients
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کلمات کلیدی
AdVCHBHBsAgHBeAgORFALTAST - آسپارتات ترانس آمینازAspartate aminotransferase - آسپارتات ترانس آمیناز یا AST Alanine aminotransferase - آلانین آمینوترانسفرازamino acid - آمینو اسیدHepatitis B surface antigen - آنتی ژن سطحی هپاتیت BReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسmutation - جهشSurface - سطحیopen reading frame - قاب خواندن بازCORE - هستهnucleos(t)ide analogues - هسته (T) ایدال آنالوگHepatitis B e antigen - هپاتیت B آنتی ژنchronic hepatitis B - هپاتیت B مزمن، هپاتیت ب مزمنHBV - هپاتیت بpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازhepatitis B virus - ویروس هپاتیت بیPolymerase - پلیمرازnuc - گردو
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
As one of the major global public health concerns, hepatitis B virus (HBV) can be divided into at least eight genotypes, which may be related to disease severity and treatment response. We previously demonstrated that genotypes B and C HBV, with distinct geographical distribution in China, had divergent genotype-dependent amino acid polymorphisms and variations in reverse transcriptase (RT) gene region, a target of antiviral therapy using nucleos(t)ide analogues. Recently recombination between HBV genotypes B and C was reported to occur in the RT region. However, their frequency and clinical significance is poorly understood. Here full-length HBV RT sequences from 201 Chinese chronic hepatitis B (CHB) patients were amplified and sequenced, among which 31.34% (63/201) were genotype B whereas 68.66% (138/201) genotype C. Although no intergenotypic recombination was detected among C-genotype HBV, 38.10% (24/63) of B-genotype HBV had recombination with genotype C in the 3â²-terminal RT sequences. The patients with B/C intergenotypic recombinants had significantly (PÂ <Â 0.05) higher serum HBV DNA level than the “pure” B-genotype cohort did. Moreover, the B/C intergenotypic recombinants were prone to more substitutions at several specific residues in the RT region than genotype B or C. Besides, unlike their parental genotypes, the recombinant HBV appeared to display an altered geographic distribution feature in China. Our findings provide novel insight into the virological, clinical and epidemiological features of new HBV B/C intergenotypic recombinants at the 3â² end of RT sequences among Chinese CHB patients. The highly complex genetic background of the novel recombinant HBV carrying new mutations affecting RT protein may contribute to an enhanced heterogeneity in treatment response or prognosis among CHB patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 57, January 2018, Pages 26-35
Journal: Infection, Genetics and Evolution - Volume 57, January 2018, Pages 26-35
نویسندگان
Baoming Liu, Jing-Xian Yang, Ling Yan, Hui Zhuang, Tong Li,