کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8648353 | 1570632 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A plant-like mitochondrial carrier family protein facilitates mitochondrial transport of di- and tricarboxylates in Trypanosoma brucei
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کلمات کلیدی
DICOxoglutarate carrierCarboxyatractylosideBSDOGCDTCTrypanosoma bruceiCITNEOTCAMCFCATROS - ROSBlasticidin - بلستیکیدینdicarboxylate carrier - حامل dicarboxylateMitochondrial transport - حمل و نقل میتوکندریMitochondrial carrier family - خانواده حامل های میتوکندریDicarboxylate - دیکربوکسیلاتEnergy metabolism - متابولیسم انرژیUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهNeomycin - نومیدینcitrate carrier - نیت سیتراتtricarboxylic acid cycle - چرخه اسید تریکاربوکیلیکReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A plant-like mitochondrial carrier family protein facilitates mitochondrial transport of di- and tricarboxylates in Trypanosoma brucei A plant-like mitochondrial carrier family protein facilitates mitochondrial transport of di- and tricarboxylates in Trypanosoma brucei](/preview/png/8648353.png)
چکیده انگلیسی
The procyclic form of the human parasite Trypanosoma brucei harbors one single, large mitochondrion containing all tricarboxylic acid (TCA) cycle enzymes and respiratory chain complexes present also in higher eukaryotes. Metabolite exchange among subcellular compartments such as the cytoplasm, the mitochondrion, and the peroxisomes is crucial for redox homeostasis and for metabolic pathways whose enzymes are dispersed among different organelles. In higher eukaryotes, mitochondrial carrier family (MCF) proteins transport TCA-cycle intermediates across the inner mitochondrial membrane. Previously, we identified several MCF members that are essential for T. brucei survival. Among these, only one MCF protein, TbMCP12, potentially could transport dicarboxylates and tricarboxylates. Here, we conducted phylogenetic and sequence analyses and functionally characterised TbMCP12 in vivo. Our results suggested that similarly to its homologues in plants, TbMCP12 transports both dicarboxylates and tricarboxylates across the mitochondrial inner membrane. Deleting this carrier in T. brucei was not lethal, while its overexpression was deleterious. Our results suggest that the intracellular abundance of TbMCP12 is an important regulatory element for the NADPH balance and mitochondrial ATP-production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 221, April 2018, Pages 36-51
Journal: Molecular and Biochemical Parasitology - Volume 221, April 2018, Pages 36-51
نویسندگان
Claudia Colasante, Fuli Zheng, Cordula Kemp, Frank Voncken,